文献类型： 外文期刊

第一作者： Wang, Jianzhong

作者： Wang, Jianzhong;Zong, Xianchun;Wang, Chunliu;Zhu, Guangmei;Yang, Guilian;Jiang, Yanlong;Yang, Wentao;Huang, Haibin;Shi, Chunwei;Zeng, Yan;Wang, Nan;Cao, Xin;Wang, Chunfeng;Wang, Jianzhong;Zong, Xianchun;Wang, Chunliu;Zhu, Guangmei;Yang, Guilian;Jiang, Yanlong;Yang, Wentao;Huang, Haibin;Shi, Chunwei;Zeng, Yan;Wang, Nan;Cao, Xin;Wang, Chunfeng;Wang, Jianzhong;Zong, Xianchun;Wang, Chunliu;Zhu, Guangmei;Yang, Guilian;Jiang, Yanlong;Yang, Wentao;Huang, Haibin;Shi, Chunwei;Zeng, Yan;Wang, Nan;Cao, Xin;Wang, Chunfeng;Liu, Lina;Feng, Na;Liu, Lina

作者机构：

关键词： bacterium-like particles; canine distemper virus; subunit vaccine; surface display technology; immune response

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2024 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Canine distemper virus (CDV) poses a severe threat to both domesticated and wild animals, including multiple carnivores. With the continued expansion of its host range, there is an urgent need for the development of a safer and more effective vaccine. In this study, we developed subunit vaccines based on a bacterium-like particle (BLP) delivery platform containing BLPs-F and BLPs-H, which display the CDV F and H glycoprotein antigens, respectively, using the antigen-protein anchor fusions produced by a recombinant baculovirus insect cell expression system. The combination of BLPs-F and BLPs-H (CDV-BLPs), formulated with colloidal manganese salt [Mn jelly (MnJ)] adjuvant, triggered robust CDV-specific antibody responses and a substantial increase in the number of interferon gamma (IFN-gamma)-secreting CD4+ and CD8+ T cells in mice. Dogs immunized intramuscularly with this vaccine not only produced CDV-specific IgG but also displayed elevated concentrations of IFN-gamma and interleukin 6 in their serum, along with an increase of the CD3+CD4+ and CD3+CD8+ T cell subsets. Consequently, this heightened immune response provided effective protection against disease development and reduced viral shedding levels following challenge with a virulent strain. These findings suggest that this BLP-based subunit vaccine has the potential to become a novel canine distemper vaccine.IMPORTANCEMany sensitive species require a safe and effective distemper vaccine. Non-replicating vaccines are preferred. We constructed subunit particles displaying canine distemper virus (CDV) antigens based on a bacterium-like particle (BLP) delivery platform. The CDV-BLPs formulated with theMn jelly adjuvant induced robust humoral and cell-mediated immune responses to CDV in mice and dogs, thereby providing effective protection against a virulent virus challenge. This work is an important step in developing a CDV subunit vaccine. Many sensitive species require a safe and effective distemper vaccine. Non-replicating vaccines are preferred. We constructed subunit particles displaying canine distemper virus (CDV) antigens based on a bacterium-like particle (BLP) delivery platform. The CDV-BLPs formulated with theMn jelly adjuvant induced robust humoral and cell-mediated immune responses to CDV in mice and dogs, thereby providing effective protection against a virulent virus challenge. This work is an important step in developing a CDV subunit vaccine.

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