Effects of 4-hydroxyphenylacetic acid, a phenolic acid compound from Yucca schidigera extract, on immune function and intestinal health in meat pigeons

文献类型： 外文期刊

第一作者： Shao, Yuxin

作者： Shao, Yuxin;Zhang, Bo;Li, Jing;Wang, Yalei;Gao, Yusheng;Ma, Jianyuan;Wang, Zheng;Gao, Yusheng;Ma, Tenghe;Li, Yipu;Xie, Shuxian;Ma, Jianyuan;Kishawy, Asmaa Taha Yaseen;Gu, Lihong

作者机构：

关键词： Yucca schidigera extract; 4-hydroxyphenylacetic acid; Meat pigeon; Gut health

期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）

ISSN： 0032-5791

年卷期： 2025 年 104 卷 10 期

页码：

收录情况： SCI

摘要： This study aimed to evaluate the effects of 4-hydroxyphenylacetic acid (4-HPAA), a phenolic acid compound from Yucca schidigera extract, in Caco-2 cells and artificial rearing of pigeons. In vitro experiments showed that 4HPAA at different levels exhibited higher cell viability than shikimic acid and 3,4-dihydroxybenzoic acid, and with the highest viability observed at 600 mu M. Additionally, 600 mu M 4-HPAA alleviated LPS-induced upregulation of IL-1 beta and CASPASE1 expressions (P < 0.05). In vivo results demonstrated that supplemental 4-HPAA at 500, 1000, and 2000 mg/kg levels did not affect the body weight of 25-day-old pigeons (P > 0.05), but all levels increased immunoglobulin G (IgG) and IgM levels (P < 0.05). Compared with control, 2000 mg/kg 4-HPAA significantly reduced serum L-lactic acid, D-lactic acid and lactate dehydrogenase (LDH) activity (P < 0.05); increased spleen and bursa of Fabricius indices and intestinal total antioxidant capacity activity of 25-day-old pigeons (P < 0.05); and elevated ileal barrier-related genes (OCCLUDIN, CLAUDIN1 and MUC2) in 7-day-old pigeons, as well as jejunal MUC2 and ileal OCCLUDIN in 25-day-old meat pigeons (P < 0.05). It also inhibited glycolytic gene hexokinase 2 expression, while upregulating the signaling pathway genes CAMK2G and MKNK1 (P < 0.05). Supplemental 500 and 1000 mg/kg 4-HPAA significantly increased serum total cholesterol levels (P < 0.05). Notably, 500 mg/kg 4-HPAA had an adverse effect on intestinal integrity, as indicated by the highest serum diamine oxidase content, and increased glycolytic gene LDH-B expression; However, it enhanced microbial richness and diversity in the intestines of 7-day-old pigeon (P < 0.05). Besides, 1000 mg/kg 4-HPAA impaired intestinal morphology, as evidenced by a lower ileal villus height-to-crypt depth ratio (P < 0.05), but resulted in the highest abundance of Lactococcus in 25-day-old pigeons. In conclusion, 600 mu M 4-HPAA exhibits antiinflammatory activity in Caco-2 cells, and 2000 mg/kg 4-HPAA promotes immune function and intestinal barrier integrity while inhibiting glycolytic gene expression and the production of related serum metabolites in meat pigeons.

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