文献类型： 外文期刊
第一作者： Jia, Kaifang
作者： Jia, Kaifang;Wang, Jinyang;Jiang, Dan;Ding, Xiangrui;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;Jia, Kaifang;Wang, Jinyang;Jiang, Dan;Ding, Xiangrui;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;Lu, Cheng
作者机构：
关键词： Antiviral; BmNPV; Bombyx mori; PAT4; Cellular autophagy; BmTORC1
期刊名称：JOURNAL OF INVERTEBRATE PATHOLOGY （ 影响因子：2.4； 五年影响因子：3.0 ）
ISSN： 0022-2011
年卷期： 2025 年 208 卷
页码：
收录情况： SCI
摘要： Proton-assisted amino acid transporter 4 (PAT4) is a member of the solute carrier (SLC) 36 family, which mediates the transmembrane transport of amino acids and their derivatives. However, the function of PAT4 in Bombyx mori is not clear. In this study, BmPAT4 was cloned and identified using PCR technology. Its open reading frame (ORF) includes 1,395 bp, encoding 464 amino acid (Aa). Moreover, the sequence of BmPAT4 has the highest similarity with wild Bombyx.mandarina, Spodoptera frugiperda and Spodoptera litura, and it has ten transmembrane domains. BmPAT4 was localized in the cell membrane and expressed in all tissues of the silkworm. After Bombyx mori nuclear polyhedrosis virus (BmNPV) infection, the expression of BmPAT4 in midgut, hemolymph and fat body was significantly up-regulated. In addition, overexpression of BmPAT4 in BmN cells could significantly inhibit the proliferation of BmNPV, and the expression of several genes in autophagy pathway decreased significantly. On the contrary, down-regulation of BmPAT4 expression by RNA interference can promote BmNPV replication and proliferation, and the expression of key genes in autophagy pathway is significantly increased. This is the first time to report that BmPAT4 has an antiviral effect in silkworm. Moreover, the silkworm activates BmTORC1 via BmPAT4, which inhibits autophagy in silkworm cells, resulting in a lack of energy and raw materials for BmNPV infection and replication in cells.
分类号：
- 相关文献
作者其他论文 更多>>
-
BmWARS inhibits BmNPV infection via the PI3K-Akt pathway
作者：Wang, Jinyang;Ding, Xiangrui;Jia, Kaifang;Chen, Haiyu;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;Wang, Jinyang;Ding, Xiangrui;Jia, Kaifang;Chen, Haiyu;Zhao, Qiaoling;Shen, Dongxu;Qiu, Zhiyong;Zhang, Xuelian;Qian, Heying;Xia, Dingguo;An, Guorong
关键词：Antiviral;
BmWARS ; BmNPV; PI3k-Akt; Cell proliferation and apoptosis -
Functional analysis of BmTsp.C in modulating infection of BmNPV through apoptosis pathways in domestic silkworm (Bombyx mori)
作者：Xu, Yuanyuan;Wei, Yimeng;Zhang, Jing;Zhang, Dan;Zhao, Qiaoling;Shen, Dongxu;Xu, Yuanyuan;Wei, Yimeng;Zhang, Jing;Zhang, Dan;Zhao, Qiaoling;Shen, Dongxu
关键词：apoptosis; BmNPV; bombyx mori; tetraspanins
-
Deciphering the preservative effects of protamine from Xinjiang Coregonus peled on grass carp (Ctenopharyngodon idellus) fillets during refrigerated storage: a perspective from the microbiome
作者：Zhang, Rong;Gao, Yanan;Zhu, Song;Gao, Lili;Zhao, Xueqin;Liu, Shuyan;Fan, Xiaoji;Wang, Tingzhang;Zhang, Rong;Fan, Yiling;Zhu, Song;Gao, Lili;Zhao, Xueqin;Jiang, Dan;Ma, Zhuang;Liu, Shuyan
关键词：Grass carp; Refrigerated storage; Protamine; Food preservative; Compound preservative; Microbiome
-
Cloning and expression analysis of a detoxification enzyme BmmGSTo3 gene in wild silkworm, Bombyx mandarina (Lepidoptera: Bombycidae)
作者：Li, Ruonan;Chen, Enxi;Zhang, Yuming;Wang, Mengjiao;Zhu, Lin;Qian, Yixuan;Zhao, Guodong;Qian, Heying;Li, Ruonan;Chen, Enxi;Zhang, Yuming;Wang, Mengjiao;Zhu, Lin;Qian, Yixuan;Zhao, Guodong;Qian, Heying
关键词：BmNPV; insecticide; oxidative stress; virus; glutathione S-transferase
-
N-cycling functional genes and soil properties shape the heterogeneity of nitrous oxide emission pathways in tea plantation soils
作者：Tu, Longping;Xu, Zhe;Yin, Huacheng;Dong, Yubing;Ma, Ruoya;Huang, Yawen;Wu, Shuang;Li, Shuqing;Liu, Shuwei;Wang, Jinyang;Han, Zhaoqiang;Zou, Jianwen;Tu, Longping;Xu, Zhe;Yin, Huacheng;Ma, Ruoya;Huang, Yawen;Wu, Shuang;Li, Shuqing;Liu, Shuwei;Wang, Jinyang;Han, Zhaoqiang;Zou, Jianwen;Tu, Longping;Xu, Zhe;Yin, Huacheng;Ma, Ruoya;Huang, Yawen;Wu, Shuang;Li, Shuqing;Liu, Shuwei;Wang, Jinyang;Han, Zhaoqiang;Zou, Jianwen;Dong, Yubing
关键词：Acid soil; Greenhouse gas; Nitrification; Denitrification; N-15 isotope
-
Pan-genome and haplotype map of cassava cultivars and wild ancestors provide insights into its adaptive evolution and domestication
作者：Xia, Zhiqiang;Zhou, Xincheng;Jiang, Sirong;Chen, Fei;Zou, Meiling;Xu, Liangye;Guo, Xin;Wang, Shujuan;Li, Mengtao;Liu, Shisheng;Bao, Yuting;Zhang, Chenji;Xiao, Jianjia;Guo, Fengguang;Shen, Xu;Li, Haozheng;Zhang, Huaifang;He, Shuang;Wan, Yinglang;Chen, Yinhua;Huang, Dongyi;Li, Bin;Peng, Ming;Chen, Xin;Wang, Wenquan;Du, Zhenglin;Chen, Meili;Zhao, Wenming;Xiao, Jingfa;Du, Zhenglin;Chen, Meili;Zhao, Wenming;Xiao, Jingfa;Zhou, Xincheng;Wang, Haiyan;Lu, Cheng;Peng, Ming;Chen, Xin;Zhu, Tingting;Wang, Le;Luo, Ming-Cheng;Qiao, Fei;Li, Kaimian;Carvalho, Luiz;Lopez-Lavalle, Luis Augusto Becerra;Zhang, Xiaofei;Ceballos, Hernan;Wang, Zhenyu;Li, Yuanchao;Zhao, Long;Yan, Huabing;Qin, Xiaochun;Ma, Ling;Zhang, Weixiong;Moller, Birger Lindberg
关键词：genome; pan-genome; structural variations; selective sweeping; evolution and domestication; cassava
-
CPF Induces GC2spd Cell Injury via ROS/AKT/Efcab6 Pathway
作者：Zhang, Xuelian;Zhang, Mengyang;Wang, Chunzhi;Song, Qingchuan;Yang, Haiyan;Tang, Qi;Pan, Chuanying;Zhang, Xuelian;Zhao, Qiaoling;Wang, Jing
关键词：Chlorpyrifos; male infertility; RNA-Sequencing; PI3K-AKT;
Efcab6
