Cloning and expression analysis of a detoxification enzyme BmmGSTo3 gene in wild silkworm, Bombyx mandarina (Lepidoptera: Bombycidae)

文献类型： 外文期刊

第一作者： Li, Ruonan

作者： Li, Ruonan;Chen, Enxi;Zhang, Yuming;Wang, Mengjiao;Zhu, Lin;Qian, Yixuan;Zhao, Guodong;Qian, Heying;Li, Ruonan;Chen, Enxi;Zhang, Yuming;Wang, Mengjiao;Zhu, Lin;Qian, Yixuan;Zhao, Guodong;Qian, Heying

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关键词： BmNPV; insecticide; oxidative stress; virus; glutathione S-transferase

期刊名称：EUROPEAN JOURNAL OF ENTOMOLOGY （ 影响因子：1.2； 五年影响因子：1.2 ）

ISSN：

年卷期： 2025 年 122 卷

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收录情况： SCI

摘要： Bombyx mandarina is one of prevalent pests in mulberry gardens during summer and autumn, posing signifi cant challenges to sericulture across China. Glutathione S-transferase (GST), a vital detoxifi cation enzyme in insects, plays a crucial role in protecting organisms from DNA damage and oxidative stress. In this study, the GSTo3 gene, coding glutathione S-transferase of B. mandarina, was cloned and its physicochemical properties and structures were predicted using bioinformatics tools. The relative expression levels in various tissues and induced transcriptional levels were detected by using qRT-PCR. Results revealed a 99.17% sequence similarity of GSTo3 gene between B. mandarina and Bombyx mori. It is mainly distributed in the head, fat body and epidermis tissues of B. mandarina larvae. After BmNPV infection, elevated GSTo3 expression levels were observed in the midgut. The transcriptional levels of GSTo3 gene were signifi cantly up-regulated after exposure to phoxim, deltamethrin and chlorfenapyr exposure respectively in the fat body and midgut of B. mandarina. These fi ndings underscore the signifi cance of GSTo3 gene in exogenous materials metabolism and provide a new perspective on the resistance mechanism in B. mandarina.

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