Functional Identification of ICE Transcription Factors in Rubber Tree

文献类型： 外文期刊

第一作者： Li, Yan

作者： Li, Yan;Quan, Caihong;Yang, Shuguang;Wu, Shaohua;Shi, Minjing;Wang, Jun;Tian, Weimin;Li, Yan;Quan, Caihong;Yang, Shuguang;Wu, Shaohua;Shi, Minjing;Wang, Jun;Tian, Weimin

作者机构：

关键词： Hevea brasiliensis Muell; Arg; ICE transcription factor; cold tolerance; real-time quantitative PCR; gene overexpression

期刊名称：FORESTS （ 影响因子：3.282； 五年影响因子：3.292 ）

ISSN：

年卷期： 2022 年 13 卷 1 期

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收录情况： SCI

摘要： ICE (inducer of CBF expression) is a positive regulator of cold signaling pathway in plants. Identification of ICE transcription factors is important for the sustainable development of the natural rubber planting industry in nontraditional regions where sudden cold waves often occur. In this study, five ICE genes were isolated from genome of rubber tree (Hevea brasiliensis Muell. Arg.) for analysing tolerance to cold stress. They shared an ICE-specific region in the highly conserved bHLH-ZIP domain and were localized in the nucleus. The HbICEs were different in transcript abundance and expression patterns in response to cold and drought stresses and among different rubber tree clones. Generally, the expression level of HbICEs was significantly higher in the cold-tolerant rubber tree clones than that in the cold-sensitive rubber tree clones. Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco, which showed a significant increase in chlorophyll content and decrease in relative water content and conductivity at the early stage of cold stress in comparison with wild-type plants. Furthermore, overexpression of HbICE2 and HbICE4, but also HbICE1 enhanced drought tolerance in transgenic Arabidopsis. The cold tolerance of rubber tree clones is positively controlled by the expression level of HbICE1, HbICE2, and HbICE4.

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