Chromosome sorting and its applications in common wheat (Triticum aestivum) genome sequencing

文献类型： 外文期刊

第一作者： Wu SuoWei

作者： Wu SuoWei;Zheng Xu;Liu BingHua;Yang Li;Song MeiFang;Zhou Peng;Zhou Yang;Meng FanHua;Wang ShanHong;Liu HongWei;Zhai HuQu;Yang JianPing;Xiao Yang;Zheng Xu;Cai YingFan;Yang JianPing;Dolezel, Jaroslav;Song MeiFang

作者机构：

关键词： Triticum aestivum; flow cytogenetics; chromosome sorting; physical map; wheat genome sequencing

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN： 1001-6538

年卷期： 2010 年 55 卷 15 期

页码：

收录情况： SCI

摘要： The large genome size (similar to 17000 Mb) and complicated DNA structures of common wheat (Triticum aestivum) hamper its genome sequencing. By means of flow cytometry, systematic investigations on individual chromosome sorting have been carried out to construct chromosome-specific bacterial artificial chromosome (BAC) libraries since the 1980s. Several wheat chromosome-specific BAC libraries, such as chromosome 3B, three D genome chromosomes (1D, 4D and 6D), and the short arm of chromosome 1B, have been developed, and the physical map of chromosome 3B was established in 2008. The same chromosome-based strategy is being employed by the International Wheat Genome Sequencing Consortium (IWGSC) to establish the physical maps of the other 20 common wheat chromosomes (cv. Chinese Spring). Several projects on wheat genome sequencing are currently underway. The availability of new sequencing technologies provides new choices for sequencing of gene space of the wheat genome. The applications of flow-sorted chromosomes in wheat genome studies present some examples to analyze the complex genomes of cereals.

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