Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69

文献类型： 外文期刊

第一作者： Li, Yinghui

作者： Li, Yinghui;Wei, Zhen-Zhen;Sela, Hanan;Govta, Liubov;Klymiuk, Valentyna;Roychowdhury, Rajib;Bocharova, Valeria;Ben-David, Roi;Pawar, Prerna B.;Jaiwar, Samidha;Fahima, Tzion;Li, Yinghui;Wei, Zhen-Zhen;Sela, Hanan;Govta, Liubov;Klymiuk, Valentyna;Roychowdhury, Rajib;Bocharova, Valeria;Ben-David, Roi;Pawar, Prerna B.;Jaiwar, Samidha;Fahima, Tzion;Wei, Zhen-Zhen;Klymiuk, Valentyna;Chawla, Harmeet Singh;Ens, Jennifer;Wiebe, Krystalee;Pozniak, Curtis J.;Klymiuk, Valentyna;Chawla, Harmeet Singh;Ens, Jennifer;Wiebe, Krystalee;Pozniak, Curtis J.;Ben-David, Roi;Zhang, Yuqi;Molnar, Istvan;Dolezel, Jaroslav;Coaker, Gitta;Chawla, Harmeet Singh;Molnar, Istvan

作者机构：

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 1 期

页码：

收录情况： SCI

摘要： Gene cloning in repeat-rich polyploid genomes remains challenging. Here, we describe a strategy for overcoming major bottlenecks in cloning of the powdery mildew resistance gene (R-gene) Pm69 derived from tetraploid wild emmer wheat. A conventional positional cloning approach was not effective owing to suppressed recombination. Chromosome sorting was compromised by insufficient purity. A Pm69 physical map, constructed by assembling Oxford Nanopore Technology (ONT) long-read genome sequences, revealed a rapidly evolving nucleotide-binding leucine-rich repeat (NLR) R-gene cluster with structural variations. A single candidate NLR was identified by anchoring RNA sequencing reads from susceptible mutants to ONT contigs and was validated by virus-induced gene silencing. Pm69 is likely a newly evolved NLR and was discovered in only one location across the wild emmer wheat distribution range in Israel. Pm69 was successfully introgressed into cultivated wheat, and a diagnostic molecular marker was used to accelerate its deployment and pyramiding with other R-genes.

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