Comparison of the Partial Structure and Antioxidant Activity of Polysaccharides from Two Species of Chinese Truffles
文献类型： 外文期刊
第一作者： Li, Xiaolin
作者： Li, Xiaolin;Ye, Lei;Kang, Zongjing;Zhang, Xiaoping;Huang, Yue;Zhang, Bo;Zhu, Zhongkai;Zou, Yuanfeng;Kang, Zongjing;Zhang, Xiaoping;Huang, Yue
作者机构：
关键词： truffle; Tuber panzhihuanense; Tuber pseudoexcavatum; polysaccharides; structure
期刊名称：MOLECULES （ 影响因子：4.411； 五年影响因子：4.587 ）
ISSN：
年卷期： 2020 年 25 卷 18 期
页码：
收录情况： SCI
摘要： Truffles are world-renowned premium commodities. Due to their unique aroma and rarity, the price of truffles has always been very high. In this study, Diethylaminoethyl anion exchange chromatography and gel filtration were employed for polysaccharide purification from two different species of Chinese truffles. Three polysaccharide fractions were obtained fromTuber panzhihuanenseand referred to as TPZ-NP, TPZ-I, and TPZ-II. Additionally, two polysaccharide fractions were purified fromT. pseudoexcavatum(TPD-NP and TPD-I). The results of structural elucidation indicated that the polysaccharide from different species showed different monosaccharide composition and linkage units, as well as molecular weight. Two of the polysaccharide fractions with the highest yield, TPZ-I and TPD-I, were chosen for biological testing. The results indicated that both fractions displayed antioxidant properties through mediation of the intestinal cellular antioxidant defense system, which could protect cultured intestinal cells from oxidative stress-induced damage and cell viability suppression. The TPD-I fraction showed stronger antioxidant effects, which may be due to the difference in structure. Further study on the structure-activity relationship is needed to be done.
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