Rapid development of attenuated IBV vaccine candidates through a versatile backbone applicable to variants

文献类型： 外文期刊

第一作者： Lu, Yuanlu

作者： Lu, Yuanlu;Zeng, Yiran;Luo, Haowei;Chen, Na;Zhao, Lingcai;Ping, Jihui;Zhang, Haitao;Xia, Jun;Xia, Jun

作者机构：

期刊名称：NPJ VACCINES （ 影响因子：6.5； 五年影响因子：6.6 ）

ISSN：

年卷期： 2025 年 10 卷 1 期

页码：

收录情况： SCI

摘要： The antigen variability of the infectious bronchitis virus (IBV) has hindered vaccine effectiveness and perpetuated its epidemic. We engineered a rapid attenuation method for IBV variants. The strategy involves creating the rH-CPDF7 backbone by recoding a segment of the H120 nonstructural protein (NSP) genome via codon pair deoptimization (CPD), facilitating S gene integration from IBV variants via transformation-associated recombination (TAR) cloning. These recombinant strains exhibited even lower pathogenicity, indicating the effectiveness of CPDF7 in reducing virulence. Importantly, the rH-CPDF7 backbone demonstrated versatility, being applicable to the development of attenuated strains for IBV variants, including the QX-type, TW-type, and GVI-type strains (different genotypes). In conclusion, our method allows for the rapid development of attenuated strains by integrating the S gene of IBV variants into the rH-CPDF7 backbone. These recombinant strains can elicit a strong immune response and provide effective protection against homologous challenges. This strategy is crucial for developing live-attenuated vaccines against emerging IBV strains.

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