Integrated cytological and transcriptomic analysis reveals insights into pollen fertility in newly synthetic Brassica allohexaploids

文献类型： 外文期刊

第一作者： Tian, Zhaoran

作者： Tian, Zhaoran;Ji, Chengyan;Xie, Zhengqing;Shi, Xinjie;Tian, Baoming;Cao, Gangqiang;Yang, Yan;Wei, Fang;Shi, Gongyao;Ji, Chengyan;Shi, Xinjie;Wei, Xiaochun

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关键词： anther; Brassica allohexaploid; chromosome segregation; pollen fertility; tapetum; meiosis; microspore development; mitosis

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： Trigenomic Brassica allohexaploids (AABBCC, 2n = 6x = 54) have great potential in oilseed breeding and genetic diversity. However, Brassica allohexaploids do not exist naturally, and the underlying mechanism regulating pollen fertility in artificially synthesized Brassica allohexaploids is still unclear. In this study, synthetic Brassica allohexaploids were produced by crossing allotetraploid B. carinata (BBCC, 2n = 4x = 34) and diploid B. rapa (AA, 2n = 2x = 20), followed by chromosome doubling. The results showed that the pollen fertility was significantly reduced and the pollen structures were mostly distorted, but the nursing anther tapetum developed normally in the synthetic Brassica allohexaploids. Furthermore, the data showed that the meiotic events occurred irregularly with uneven chromosome segregation and microspore development appeared mostly abnormal. Transcription analysis showed that the upregulation of genes related to the negative regulation of flower development and the downregulation of genes related to chromosome segregation might play an essential role in reduction of pollen fertility in the Brassica allohexaploids. In conclusion, this study elucidated the related mechanisms affecting pollen fertility during male gametophytic development at the cytological and transcriptomic levels in the newly synthesized Brassica allohexaploids.

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