Physiological Functions of the Cello-Oligosaccharides Binding CebE in the Pathogenic Streptomyces sp. AMCC400023

文献类型： 外文期刊

第一作者： Li, Qiuyue

作者： Li, Qiuyue;Chang, Jiawen;Li, Junxia;Duan, Yuxia;Tian, Dandan;Ge, Fei;Su, Gaoya;Gao, Zheng;Zhou, Bo;Lv, Peiwen;Yang, Chunyu;Nie, Fengjie

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关键词： pathogenic Streptomyces; cello-oligosaccharides binding protein; binding affinity; sporulation

期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）

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年卷期： 2024 年 12 卷 3 期

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收录情况： SCI

摘要： Potato common scab, an economically important disease worldwide, is caused by pathogenic Streptomyces strains mainly through the effects of thaxtomin. The cello-oligosaccharides binding protein CebE is proposed as a gateway to the pathogenic development of Streptomyces scabiei. In this study, two functional CebE encoding genes, GEO5601 and GEO7671, were identified in pathogenic Streptomyces sp. AMCC400023. With a higher binding affinity towards signal molecules, the deletion of GEO5601 severely impaired thaxtomin-producing capacity and reduced the strain's pathogenicity. Transcriptional analysis confirmed that CebE(5601) is also responsible for the import and provision of carbon sources for cell growth. With lower binding affinity, the pathogenicity island (PAI)-localized CebE(7671) may assume a new function of mediating the biological process of sporulation, given the significantly impaired formation of Delta GEO7671 spores. The mechanisms of action of CebE proteins unraveled in Streptomyces sp. AMCC400023 will help pave the way for more effective prevention of the potato common scab disease.

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