Untargeted and targeted metabolomics identify metabolite biomarkers for Salmonella enteritidis in chicken meat

文献类型： 外文期刊

第一作者： Chen, Lan

作者： Chen, Lan;Ding, Hao;Gao, Yushi;Zhu, Yali;Guo, Yawen;Tang, Yayun;Xie, Kaizhou;Zhang, Genxi;Dai, Guojun;Zhang, Tao;Chen, Lan;Ding, Hao;Zhu, Yali;Guo, Yawen;Tang, Yayun;Xie, Kaizhou;Zhang, Genxi;Dai, Guojun;Zhang, Tao;Gao, Yushi

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关键词： Salmonella Enteritidis; Chicken; Metabolomics; Metabolite biomarkers

期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）

ISSN： 0308-8146

年卷期： 2023 年 409 卷

页码：

收录情况： SCI

摘要： Salmonella Enteritidis easily contaminate chicken during slaughtering, processing, transportation, and sales, which seriously endangers human health. This study aimed to identify metabolite biomarkers for Salmonella Enteritidis contamination in chicken meat. UPLC-Q-Orbitrap MS untargeted metabolomics analysis identified 441 and 240 confidently metabolites in positive and negative ion mode, respectively. Thirty metabolites were defined as potential biomarkers for Salmonella enteritidis contamination in chicken meat. UPLC-QQQ-MS based targeted metabolomics was used to quantitatively analyze candidate metabolite biomarkers in Salmonella enteritidis contaminated and fresh chicken samples. A total of 10 candidate metabolite biomarkers were confirmed in the validation set, among which acetylcholine, L-Methionine, L-Proline, L-Valine, and L-Norleucine were identified as biomarkers for Salmonella Enteritidis contamination in chicken. The combined receiver operating characteristic curve analysis of the five biomarkers achieved an AUC of 0.956, indicating their high sensitivity and specificity in predicting Salmonella Enteritidis in raw chicken. In conclusion, the present study identified five metabolite biomarkers for Salmonella enteritidis in raw chicken. These results provide a potential theoretical basis for developing Salmonella Enteritidis detection methods in raw chicken.

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