Identification and utilization of a novel large-effect QTL and linked markers for enhancing protein concentration in peanut

文献类型： 外文期刊

第一作者： Wang, Mingjun

作者： Wang, Mingjun;Guo, Jianbin;Jin, Gaorui;Yang, Taihua;Chen, Weigang;Chen, Yuning;Huang, Li;Luo, Huaiyong;Zhou, Xiaojing;Liao, Boshou;Jiang, Huifang;Liu, Nian;Lei, Yong

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0040-5752

年卷期： 2025 年 138 卷 9 期

页码：

收录情况： SCI

摘要： Peanut is a vital source of protein for humans, playing a key role in maintaining a steady protein supply. In this study, the high-protein cultivar Zhonghua6 (29.14 +/- 1.69%) was crossed with Xuhua13 (24.55 +/- 1.84%) to construct a recombinant inbred line (RIL) population. The protein concentration of the RIL population exhibited significant variation, ranging from 21.05 to 30.28%. To discover genomic regions associated with protein concentration, four libraries were constructed (two parents and two extreme bulks) for bulked segregant sequencing (BSA-seq). The results revealed significant associations between protein concentration and the genomic regions on chromosomes A07, A10, B03, and B10. Through linkage analysis, nine QTLs (quantitative trait loci) for protein concentration were identified, among which the large-effect and stable QTL qPCB03 on chromosome B03 (125.92-127.94 Mb) explained 11.03-12.86% of the phenotypic variation. This QTL (qPCB03), simultaneously identified by both BSA-Seq and linkage mapping, had not been documented in prior studies. Within the similar to 2 Mb interval of qPCB03, a total of 349 genomic variants were discovered, including six single nucleotide polymorphisms (SNPs) that resulted in nonsynonymous mutations in six genes. Based on genomic variants of the target region, KASP markers were developed and validated in 147 peanut varieties exhibiting variable seed protein concentration. According to the marker-assisted selection, favorable genotypes could potentially enhance protein concentration by 1.23-1.57 percentage points in the RIL populations. The identification of stable loci and the development of markers facilitate marker-assisted breeding in peanuts, while the discovery of candidate genes lays the groundwork for the fine mapping of key genes regulating protein concentration.

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