Dynamic metabolite QTL analyses provide novel biochemical insights into kernel development and nutritional quality improvement in common wheat

文献类型： 外文期刊

第一作者： Yin, Bo

作者： Yin, Bo;Jia, Jingqi;Sun, Xu;Hu, Xin;Ao, Min;Liu, Wei;Tian, Zhitao;Liu, Hongbo;Li, Dongqin;Chen, Jie;Chen, Wei;Yin, Bo;Jia, Jingqi;Sun, Xu;Hu, Xin;Ao, Min;Liu, Wei;Tian, Zhitao;Liu, Hongbo;Li, Dongqin;Chen, Jie;Chen, Wei;Yin, Bo;Jia, Jingqi;Sun, Xu;Hu, Xin;Ao, Min;Liu, Wei;Tian, Zhitao;Chen, Jie;Chen, Wei;Tian, Wenfei;Hao, Yuanfeng;Xia, Xianchun;He, Zhonghu;Sade, Nir;Brotman, Yariv;Fernie, Alisdair R.;Chen, Jie

作者机构：

关键词： mQTL; wheat kernel development; gene validation; nutritional quality; wheat

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 5 期

页码：

收录情况： SCI

摘要： Despite recent advances in crop metabolomics, the genetic control and molecular basis of the wheat kernel metabolome at different developmental stages remain largely unknown. Here, we performed widely targeted metabolite profiling of kernels from three developmental stages (grain -filling kernels [FKs], mature kernels [MKs], and germinating kernels [GKs]) using a population of 159 recombinant inbred lines. We detected 625 annotated metabolites and mapped 3173, 3143, and 2644 metabolite quantitative trait loci (mQTLs) in FKs, MKs, and GKs, respectively. Only 52 mQTLs were mapped at all three stages, indicating the high stage specificity of the wheat kernel metabolome. Four candidate genes were functionally validated by in vitro enzymatic reactions and/or transgenic approaches in wheat, three of which mediated the tricin metabolic pathway. Metabolite flux efficiencies within the tricin pathway were evaluated, and superior candidate haplotypes were identified, comprehensively delineating the tricin metabolism pathway in wheat. Finally, additional wheat metabolic pathways were re -constructed by updating them to incorporate the 177 candidate genes identified in this study. Our work provides new information on variations in the wheat kernel metabolome and important molecular resources for improvement of wheat nutritional quality.

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