Development of 68 SSR markers for Hemibagrus guttatus, a protected indigenous catfish in China

文献类型： 外文期刊

第一作者： Yang, Yexin

作者： Yang, Yexin;Liu, Yi;Song, Jiahuan;Liu, Chao;Mu, Xidong;Yang, Yexin;Zhu, Shandian;Xiong, Bingyuan;Wu, Yuli

作者机构：

关键词： SSR markers; Hemibagrus guttatus; Genetic diversity; Whole-genome resequencing

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.9； 五年影响因子：0.9 ）

ISSN： 1877-7252

年卷期： 2025 年 17 卷 1 期

页码：

收录情况： SCI

摘要： Hemibagrus guttatus (Lacep & egrave;de, 1803), commonly known as spotted longbarbel catfish, is an indigenous catfish in China. However, due to the sharp decrease of H. guttatus wild populations, it is assigned as "Population Decreasing" by IUCN and is a second-level National Key Protected Wildlife species in China. It is well known that genetic diversity is crucial for animals' protection. However, there are limited molecular markers for H. guttatus. In this study we thus developed 68 simple sequence repeat (SSR) markers in this study based on the whole genome resequencing of 20 individuals. The minor allele frequency values were between 0.08 and 0.50. The observed heterozygosity ranged from 0.33 to 0.75, and the expected heterozygosity ranged from 0.33 to 0.80. Eight loci were significantly deviated from the Hardy-Weinberg equilibrium (p < 0.05). SSR markers developed in the present study will be helpful for the population genetics of H. guttatus.

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