Gravity-driven and rotation-controlled microfluidic chip for point-of-care nucleic acid detection in the fully closed environment

文献类型： 外文期刊

第一作者： Chen, Yanju

作者： Chen, Yanju;Zhu, Yuanyuan;Wu, Jian;Wang, Xiaofu;Peng, Cheng;Xu, Junfeng;Wang, Rui;Wang, Tingzhang;Che, Yang;Chen, Yanju;Zhu, Yuanyuan;Wu, Jian

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关键词： Microfluidic chip; Closed environment; Gravity; Rotation; Point-of-care; Self-testing

期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）

ISSN： 0039-9140

年卷期： 2024 年 267 卷

页码：

收录情况： SCI

摘要： Point-of-care nucleic acid detection is essential for diagnosis and food safety, especially in resource-limited areas. This study reports a g ravity-driven and r otation-controlled (GR) chip-coupled lateral flow-based assay (LFA) for point-of-care nucleic acid detection. The sample solution is added to the inlet of the GR chip and flows into the loop-medicated isothermal amplification (LAMP) chamber by the action of gravity. After the LAMP reaction, the GR chip is manually rotated to allow the amplified solution to flow into the LFA chamber for result readout. The GR chip integrates the LAMP reaction and LFA in a fully closed environment, avoiding the aerosol contamination problem. The GR chip's simple and ingenious structure simplifies the fabrication and reduces its cost, making it possible for point-of-care and even home self-testing.

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