Establishment of a genetic transformation system for maize inbred P9-10

文献类型： 外文期刊

第一作者： Zhou, FY

作者： Zhou, FY;Wang, GY;Xie, YJ;Cui, HZ;Guo, SD;Dai, JR

作者机构：

关键词： maize;inbred;genetic transformation

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

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年卷期：

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收录情况： SCI

摘要： It was found that the supplement of 10~-4 mol/L AgNO_3 in N6medium enhanced the induction of typeⅠembryogenic calli fromimmature embryos of maize inbred P9-10. After high-osmotic treatment,the induced calli was taken as transformation recipient to bebombarded with plasmid pMG6 carrying synthetic Bt gene by PDS-100/Hegenegum. A total of 14 resistant calli were obtained after screeningsubsequently on the mediums with gradually increasing selec- tivepressure.

分类号： N1

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