SSR marker-based mapping and linkage analysis of Bombyx mori thermotolerance gene

文献类型： 外文期刊

第一作者： Zhao, Yuan

作者： Zhao, Yuan;Wu, Yang-Chun;Zhu, Ya-Hong;Zhao, Yuan;Zhang, Jiang

作者机构：

关键词： backcrosses;gene expression;genes;genetic mapping;genetic markers;linkage;silkworms;simple sequence repeats;strains;tolerance;Bombyx mori

期刊名称：JOURNAL OF FOOD AGRICULTURE & ENVIRONMENT （ 影响因子：0.435； 五年影响因子：0.484 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Based on the characteristic that there is crossing-over in male Bombyx mori but no crossing-over in female Bombyx mori, Dong34, a thermotolerant strain, and Ou17, a highly susceptible strain, were used to produce two backcross populations, namely, (Ou17 X Dong34)female X Ou17 male and Ou17 female X (Ou17 X Dong34)male, which were designated as BC1F and BC1M, respectively. With 84 specific primers for molecular markers selected from published SSR marker-based molecular linkage map, twenty two BC IF individuals were used to screen simple sequence repeat (SSR) molecular markers linked to thermotolerance gene (KN), and 5 SSR molecular markers on the 8(th) linkage group were found to link to KN. Subsequently, 192 BC I M individuals were used to map these markers and KN, and a linkage map was constructed, where the order of the five SSR molecular markers and KN are LFL1123, LFL0407, KN, LFL0329, LFL0658 and LFL0944.

分类号： TS2

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