Genetic and transcriptome analysis of the smb mutant in Bombyx mori

文献类型： 外文期刊

第一作者： Liu, Weibin

作者： Liu, Weibin;Huang, Tianchen;Deng, Gang;Mei, Xinglin;Zhong, Shanshan;Shen, Dongxu;Zhang, Xuelian;Jiang, Li;Zhao, Qiaoling;Shen, Dongxu;Zhang, Xuelian;Zhao, Qiaoling

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关键词： Bombyx mori; RNA-Seq; smb mutant; Transcriptome

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：3.0； 五年影响因子：3.2 ）

ISSN： 1744-117X

年卷期： 2024 年 49 卷

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收录情况： SCI

摘要： More than 600 mutations have been discovered in the history of silkworm domestication. It is important to study the formation mechanism of these mutations to further understand the life and development process of silk-worms and agricultural pest control. The silkworm mutant smb was isolated from silkworm strain NCV, and transcriptome analysis was performed on the silkworm mutant. 796 differentially expressed genes (DEGs) were detected at 48 h of the second instar stage with 669 genes significantly upregulated and 127 genes significantly downregulated. During the GO enrichment analysis, it was found that the enrichment of biological processes was mainly concentrated in proteolysis, carbohydrate metabolism, aminoglycan metabolism, organic substance metabolism, protein metabolism and so on. Based on the analysis of KEGG pathways, it revealed that the pathways enriched in lysosomes, AMPK signaling, fatty acid metabolism, PPAR signaling, galactose metabolism, and protein digestion and absorption were the most significant. Through these most significantly enriched GO terms and KEGG pathways, DEGs consistent with the phenotypic characteristics of the smb mutant were identified, including small body size, slow development, and successive death after the fourth instar. These results provided experimental evidence for the potential formation mechanism of smb mutants.

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