Silkworm pupae protein co-degradation by magnetic nanoparticles immobilized proteinase K and Mucor circinelloides aspartic protease for further utilization of sericulture by-products

文献类型： 外文期刊

第一作者： Zhu, Xuan

作者： Zhu, Xuan;Zhao, Yi-Fan;Wen, Hong-Jian;Lu, Yu;You, Shuai;Herman, Richard Ansah;Wang, Jun;You, Shuai;Herman, Richard Ansah;Wang, Jun

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关键词： Silkworm pupae protein; Magnetic Janus mesoporous silica; nanoparticles; Immobilized proteinase K; Immobilized Mucor circinelloides aspartic; protease; Co -degradation

期刊名称：ENVIRONMENTAL RESEARCH （ 影响因子：8.3； 五年影响因子：8.2 ）

ISSN： 0013-9351

年卷期： 2024 年 249 卷

页码：

收录情况： SCI

摘要： Silkworm pupae, by-product of sericulture industry, is massively discarded. The degradation rate of silkworm pupae protein is critical to further employment, which reduces the impact of waste on the environment. Herein, magnetic Janus mesoporous silica nanoparticles immobilized proteinase K mutant T206M and Mucor circinelloides aspartic protease were employed in the co-degradation. The thermostability of T206M improved by enhancing structural rigidity (t(1/2) by 30 min and T-50 by 5 C-degrees), prompting the degradation efficiency. At 65 C and pH 7, degradation rate reached the highest of 61.7%, which improved by 26% compared with single free protease degradation. Besides, the immobilized protease is easy to separate and reuse, which maintains 50% activity after 10 recycles. Therefore, immobilized protease co-degradation was first applied to the development and utilization of silkworm pupae resulting in the release of promising antioxidant properties and reduces the environmental impact by utilizing a natural and renewable resource.

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