Antigenic and immunogenic properties of recombinant proteins consisting of two immunodominant African swine fever virus proteins fused with bacterial lipoprotein OprI

文献类型： 外文期刊

第一作者： Zhang, Guanglei

作者： Zhang, Guanglei;Liu, Wei;Gao, Zhan;Chang, Yanyan;Yang, Sicheng;Peng, Qian;Ge, Sudan;Kang, Bijing;Shao, Junjun;Chang, Huiyun

作者机构：

关键词： African swine fever virus; Immunomodulation; Immune response; OprI; Recombinant fusion proteins; Vaccine

期刊名称：VIROLOGY JOURNAL （ 影响因子：5.913； 五年影响因子：4.372 ）

ISSN：

年卷期： 2022 年 19 卷 1 期

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收录情况： SCI

摘要： Background African swine fever (ASF) is a highly fatal swine disease, which threatens the global pig industry. There is no commercially available vaccine against ASF and effective subunit vaccines would represent a real breakthrough. Methods In this study, we expressed and purified two recombinant fusion proteins, OPM (OprI-p30-modified p54) and OPMT (OprI-p30-modified p54-T cell epitope), which combine the bacterial lipoprotein OprI with ASF virus proteins p30 and p54. Purified recombinant p30 and modified p54 expressed alone or fused served as controls. The activation of dendritic cells (DCs) by these proteins was first assessed. Then, humoral and cellular immunity induced by the proteins were evaluated in mice. Results Both OPM and OPMT activated DCs with elevated expression of relevant surface molecules and proinflammatory cytokines. Furthermore, OPMT elicited the highest levels of antigen-specific IgG responses, cytokines including interleukin-2, interferon-gamma, and tumor necrosis factor-alpha, and proliferation of lymphocytes. Importantly, the sera from mice vaccinated with OPM or OPMT neutralized more than 86% of ASF virus in vitro. Conclusions Our results suggest that OPMT has good immunostimulatory activities and immunogenicity in mice, and might be an appropriate candidate to elicit immune responses in swine. Our study provides valuable information on further development of a subunit vaccine against ASF.

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