Novel Arginine End-Tagging Antimicrobial Peptides to Combat Multidrug-Resistant Bacteria

文献类型： 外文期刊

第一作者： Shang, Lu

作者： Shang, Lu;Wu, Yuting;Wei, Nan;Yang, Fayu;Wang, Mi;Zhang, Lifang;Fei, Chenzhong;Liu, Yingchun;Xue, Feiqun;Gu, Feng;Shang, Lu;Wu, Yuting;Wei, Nan;Yang, Fayu;Wang, Mi;Zhang, Lifang;Fei, Chenzhong;Liu, Yingchun;Xue, Feiqun;Gu, Feng

作者机构：

关键词： antimicrobial; arginine end-tagging peptides; multidrug-resistant bacteria; biofilm; biocompatibility

期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：10.383； 五年影响因子：10.382 ）

ISSN： 1944-8244

年卷期： 2022 年 14 卷 1 期

页码：

收录情况： SCI

摘要： The emergence of multidrug-resistant microorganisms has been termed one of the most common global health threats, emphasizing the discovery of new antibacterial agents. To address this issue, we engineered peptides harboring "RWWWR" as a central motif plus arginine (R) end-tagging and then tested them in vitro and in vivo. Our results demonstrate that Pep 6, one of the engineered peptides, shows great potential in combating Escherichia coli bacteremia and the Staphylococcus aureus skin burn infection model, which induces a 62-90% reduction in bacterial burden. Remarkably, after long serial passages of S. aureus and E. coli for 30 days, Pep 6 is still highly efficient in killing pathogens, compared with 64- and 128-fold increase in minimal inhibitory concentrations (MICs) for vancomycin and polymyxin B, respectively. We also found that Pep 6 exhibited robust biofilm-inhibiting activity and eliminated 61.33% of the mature methicillin-resistant Staphylococcus aureus (MRSA) biofilm with concentration in the MIC level. These results suggest that the RWWWR motif and binding of arginine end-tagging could be harnessed as a new agent for combating multidrug-resistant bacteria.

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