Nitrogen metabolism of the highly ureolytic bacterium Proteus penneri S99 isolated from the rumen

文献类型： 外文期刊

第一作者： Liu, Sijia

作者： Liu, Sijia;Zheng, Nan;Wang, Jiaqi;Zhao, Shengguo;Liu, Sijia

作者机构：

关键词： Rumen; Urease; Proteus penneri S99; Ammonia assimilation; Transcriptome

期刊名称：BMC MICROBIOLOGY （ 影响因子：4.2； 五年影响因子：4.8 ）

ISSN： 1471-2180

年卷期： 2025 年 25 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe model rumen-dominant ureolytic bacterium P. penneri S99 exhibits high urease activity. It was cultivated using ammonia, urea, amino acids, or their combination as nitrogen sources. To identify differences in gene expression, the transcript abundances of various genes involved in nitrogen metabolism were analyzed by harvesting mRNA from cells during the exponential growth phases on different nitrogen sources.ResultsP. penneri S99 can utilize ammonia, urea, or amino acids as the sole nitrogen sources for growth and shows a preference for utilizing urea. It exhibits similar growth rates and maximum biomass on ammonia and urea, but showed higher growth rates and maximum biomass on amino acids. Transcriptome sequencing analysis revealed different transcription patterns in response to different nitrogen sources. The urease gene expression was detected in all three different nitrogen sources, and complete hydrolysis of urea was also observed when other nitrogen sources were added to the medium containing urea. The regulation of urease in P. penneri S99 was characterized by constitutive expression, not by urea. The growth of P. penneri S99 on ammonia, ammonium acid, and urea was similar, with the only observed difference being an increase in urease transcript abundance.ConclusionsThe transcription patterns of nitrogen metabolism genes offer insights into how nitrogen is utilized in the rumen.

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