Proteomic Identification of Differentially Expressed Proteins in the Ligon lintless Mutant of Upland Cotton (Gossypium hirsutum L.)

文献类型: 外文期刊

第一作者: Zhao, Pi-Ming

作者: Zhao, Pi-Ming;Wang, Li-Li;Han, Li-Bo;Wang, Juan;Yao, Yuan;Wang, Hai-Yun;Luo, Yuan-Ming;Xia, Gui-Xian;Zhao, Pi-Ming;Wang, Li-Li;Han, Li-Bo;Wang, Juan;Yao, Yuan;Wang, Hai-Yun;Xia, Gui-Xian;Zhao, Pi-Ming;Wang, Li-Li;Han, Li-Bo;Wang, Juan;Yao, Yuan;Wang, Hai-Yun;Xia, Gui-Xian;Du, Xiong-Ming

作者机构:

关键词: proteomics;cotton fiber;Ligon mutant;cytoskeleton;cell elongation

期刊名称:JOURNAL OF PROTEOME RESEARCH ( 影响因子:4.466; 五年影响因子:4.352 )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: Cotton fiber is an ideal model for studying plant cell elongation. To date, the underlying mechanisms controlling fiber elongation remain unclear due to their high complexity. In this study, a comparative proteomic analysis between a short-lint fiber mutant (Ligon lintless, Li-1) and its wild-type was performed to identify fiber elongation-related proteins. By 2-DE combined with local EST data base-assisted MS/MS analysis, 81 differentially expressed proteins assigned to different functional categories were identified from Li-1 fibers, of which 54 were down-regulated and 27 were up-regulated. Several novel aspects regarding cotton fiber elongation can be illustrated from our data. First, over half of the down-regulated proteins were newly identified at the protein level, which is mainly involved in protein folding and stabilization, nucleocytoplasmic transport, signal transduction, and vesicular-mediated transport. Second, a number of cytoskeleton-related proteins showed a remarkable decrease in protein abundance in the Li-1 fibers. Accordingly, the architecture of actin cytoskeleton was severely deformed and the micrbtubule organization was moderately altered, accompanied with dramatic disruption of vesicle trafficking. Third, the expression of several proteins involved in unfolded protein response (UPR) was activated in Li-1 fibers, indicating that the deficiency of fiber cell elongation was related to ER stress. Collectively, these findings significantly advanced our understanding of the mechanisms associated with cotton fiber elongation.

分类号: Q7

  • 相关文献

[1]Rapid auxin-mediated changes in the proteome of the epidermal cells in rye coleoptiles: implications for the initiation of growth. Deng, Z.,Xu, S.,Wang, Z. -Y.,Kutschera, U.,Deng, Z.,Xu, S.,Chalkley, R. J.,Oses-Prieto, J. A.,Burlingame, A. L..

[2]Effects of lipopolysaccharide on maturation of bovine oocyte in vitro and its possible mechanisms. Zhao, Shan-Jiang,Pang, Yun-Wei,Zhao, Xue-Ming,Du, Wei-Hua,Hao, Hai-Sheng,Zhu, Hua-Bin. 2017

[3]The plant cytoskeleton controls regulatory volume increase. Liu, Qiong,Ismail, Ahmed,Chang, Xiaoli,Nick, Peter,Qiao, Fei.

[4]Cotton Ascorbate Oxidase Promotes Cell Growth in Cultured Tobacco Bright Yellow-2 Cells through Generation of Apoplast Oxidation. Li, Rong,Xin, Shan,Tao, Chengcheng,Li, Hongbin,Jin, Xiang. 2017

[5]Effect of GbKTN1 from Gossypium barbadense on cell elongation of fission yeast (Schizosaccharomyces pombe). Li, WM,Wang, ZX,Jia, SR.

[6]DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation. Xu, Zongchang,Wang, Meng,Shi, Dachuan,Niu, Tiantian,Kong, Yingzhen,Xu, Zongchang,Wang, Meng,Zhou, Gongke,Hahn, Michael G.,O'Neill, Malcolm A.,Hahn, Michael G..

[7]GhVLN4 is involved in cell elongation via regulation of actin organization. Fenni Lv,Liu, Kang,Yuan, Youlu,Mingya Han,Dongdong Ge,Hui Dong,Xiaotong Zhang,Lifeng Li,Peipei Zhang,Zhongqi Zhang,Jing Sun,Kang Liu,Youlu Yuan.

[8]Mutation of Rice BC12/GDD1, Which Encodes a Kinesin-Like Protein That Binds to a GA Biosynthesis Gene Promoter, Leads to Dwarfism with Impaired Cell Elongation. Li, Juan,Jiang, Jiafu,Xu, Yunyuan,Zhang, Cui,Xiao, Jun,Du, Cheng,Luo, Wei,Chong, Kang,Li, Juan,Zhang, Cui,Xiao, Jun,Du, Cheng,Qian, Qian,Zou, Guoxing,Chen, Mingluan,Huang, Yunqing,Feng, Yuqi,Cheng, Zhukuan,Yuan, Ming,Chong, Kang.

[9]GNS4, a novel allele of DWARF11, regulates grain number and grain size in a high-yield rice variety. Zhou, Yong,Tao, Yajun,Miao, Jun,Liu, Jun,Liu, Yanhua,Yi, Chuandeng,Yang, Zefeng,Gong, Zhiyun,Liang, Guohua,Zhu, Jinyan. 2017

[10]Natural Variations in SLG7 Regulate Grain Shape in Rice. Miao, Jun,Peng, Xiurong,Leburu, Mamotshewa,Yuan, Fuhai,Gu, Houwen,Gao, Yun,Tao, Yajun,Gong, Zhiyun,Yi, Chuandeng,Gu, Minghong,Yang, Zefeng,Liang, Guohua,Gu, Haiyong,Zhu, Jinyan.

[11]A Cotton BURP Domain Protein Interacts With -Expansin and Their Co-Expression Promotes Plant Growth and Fruit Production. Bing Xu,Jin-Ying Gou,Fu-Guang Li,Xiao-Xia Shangguan,Bo Zhao,Chang-Qing Yang,LingJian Wang,Sheng Yuan,Chang-Jun Liu,Xiao-Ya Chen. 2013

[12]Molecular Cloning, and Characterization of an Adenylyl Cyclase-Associated Protein from Gossypium arboreum L.. Wang Sheng,Zhao Guo-hong,Jia Yin-hua,Du Xiong-ming. 2009

[13]MODIFIED METHODS FOR THE ANALYSIS OF THE LIGNIN-LIKE PHENOLIC POLYMER CONTENTS OF COTTON FIBERS. Hu, Wen-Ran,Tian, Xiao-Li,Xie, Li-Xia,Hu, Wen-Ran,Fan, Ling,Xie, Li-Xia. 2015

[14]Levels of cytokinins in the ovules of cotton mutants with altered fiber development. Chen, JG,Du, XM,Zhou, X,Zhao, HY. 1997

[15]Co-expression and preferential interaction between two calcineurin B-like proteins and a CBL-interacting protein kinase from cotton. Gao, Peng,Zhao, Pi-Ming,Wang, Juan,Wang, Hai-Yun,Wang, Gui-Ling,Xia, Gui-Xian,Gao, Peng,Zhao, Pi-Ming,Wang, Juan,Wang, Hai-Yun,Wang, Gui-Ling,Xia, Gui-Xian,Du, Xiong-Ming.

[16]Overexpression of a Profilin (GhPFN2) Promotes the Progression of Developmental Phases in Cotton Fibers. Wang, Juan,Wang, Hai-Yun,Zhao, Pi-Ming,Han, Li-Bo,Xia, Gui-Xian,Wang, Juan,Wang, Hai-Yun,Zhao, Pi-Ming,Han, Li-Bo,Xia, Gui-Xian,Wang, Juan,Wang, Hai-Yun,Zhao, Pi-Ming,Han, Li-Bo,Xia, Gui-Xian,Jiao, Gai-Li,Zheng, Yi-Yan,Huang, Shan-Jin. 2010

[17]Molecular and Biochemical Evidence for Phenylpropanoid Synthesis and Presence of Wall-linked Phenolics in Cotton Fibers. Fan, Ling,Hu, Wen-Ran,Hao, Xiao-Yan,Wang, Dong-Mei,Yuan, Hui,Yan, Hong-Ying,Shi, Wei-Jun. 2009

[18]Functional Analysis of Cotton DELLA-Like Genes that are Differentially Regulated during Fiber Development. Wen, Wei,Yu, Xiaoling,Chen, Qiang,Peng, Ming,Wen, Wei,Yu, Xiaoling,Chen, Qiang,Peng, Ming,Cui, Baiming,Zheng, Yinying,Peng, Ming,Xia, Yiji.

[19]Cloning and characterization of GbGI, a DELLA-like gene from cotton (Gossypium barbadense). Yu, Xiaoling,Ruan, Mengbin,Wen, Wei,Wang, Shuchang,Peng, Ming,Yu, Xiaoling,Cui, Baiming,Di, Rong.

[20]iTRAQ-proteomics and bioinformatics analyses of mammary tissue from cows with clinical mastitis due to natural infection with Staphylococci aureus. Huang, Jinming,Luo, Guojing,Zhang, Zijing,Wang, Xiuge,Ju, Zhihua,Qi, Chao,Zhang, Yan,Wang, Changfa,Li, Rongling,Li, Jianbin,Yin, Weijun,Zhong, Jifeng,Luo, Guojing,Zhang, Zijing,Xu, Yinxue,Moisa, Sonia J.,Loor, Juan J.,Loor, Juan J.,Moisa, Sonia J.,Loor, Juan J.. 2014

作者其他论文 更多>>