Multi-omics analysis of the metabolism of phenolic compounds in tea leaves by Aspergillus luchuensis during fermentation of pu-erh tea br

文献类型： 外文期刊

第一作者： Ma, Yan

作者： Ma, Yan;Jiang, Bin;Liu, Kunyi;Li, Ruoyu;Chen, Lijiao;Liu, Zhiwei;An, Jiangshan;Luo, Hui;Lv, Caiyou;Ling, Tiejun;Zhao, Ming;Ma, Yan;Jiang, Bin;Liu, Kunyi;Li, Ruoyu;Chen, Lijiao;Liu, Zhiwei;An, Jiangshan;Luo, Hui;Lv, Caiyou;Zhao, Ming;Ling, Tiejun;Zhao, Ming;Wu, Jianlin;Xiang, Guisheng;Zhao, Ming;Xiang, Guisheng;Jiang, Bin;Liu, Kunyi;Jiang, Bin;Liu, Kunyi;Pan, Yinghong

作者机构：

关键词： Aspergillus; Fermentation; Mycotoxins; Phenolic compound; Pu-erh tea

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）

ISSN： 0963-9969

年卷期： 2022 年 162 卷

页码：

收录情况： SCI

摘要： Aspergillus fungi are extensively used in traditional food fermentation, so their functions, mechanisms, and safety risks are worth exploring. In this study, a dominant fungal strain (P1) was isolated from a fermented pu-erh tea and identified as A. luchuensis by phylogenetic analysis of fungal internally-transcribed spacer sequencing, partial beta-tubulin and calmodulin genes. A pure-strain fermentation of tea leaves was developed, and tea compounds were analyzed by widely-targeted metabolomics, using high-performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC-MS). The mycotoxins, aflatoxin (B1, B2, M1 and M2), fumonisin B1 and B2, ochratoxin A, citrinin, were not detected in fermented tea leaves using methods in the National Standard of the Peoples' Republic of China. The genome of 36.60 Mb with 11,836 protein-coding genes was sequenced by PacBio sequencing and annotated. Expression of fungal genes during fermentation was analyzed by Illumina HiSeq 2500; genes encoding enzymes including glycoside hydrolases, phenolic acid esterases, laccases, tyrosinases, dehydrogenases, peroxidases, dioxygenases, monooxygenases, decarboxylases and O-methyltransferases were identified. These enzymes catalyze hydrolysis, oxidation, ring cleavage, hydroxylation, decarboxylation and O-methylation of phenolic compounds , significantly (p < 0.05) changing the phenolic compound composition. While, phenolic compounds were degraded through degradation of aromatic compounds pathways and xenobiotics biodegradation and metabolism pathways. These findings advance knowledge of the functions and mechanisms of action of Aspergillus in traditional food fermentation.

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