Preparation of Specific Nanobodies and Their Application in the Rapid Detection of Nodularin-R in Water Samples

文献类型： 外文期刊

第一作者： Yang, Jinyi

作者： Yang, Jinyi;Si, Rui;Wu, Guangpei;Fang, Ruyu;Liu, Fei;Wang, Feng;Lei, Hongtao;Shen, Yudong;Wang, Hong;Wang, Yu;Zhang, Qi

作者机构：

关键词： nodularin; nanobody; detection; ic-ELISA

期刊名称：FOODS （ 影响因子：5.561； 五年影响因子：5.94 ）

ISSN：

年卷期： 2021 年 10 卷 11 期

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收录情况： SCI

摘要： Nanobodies have several advantages, including great stability, sensibility, and ease of production; therefore, they have become important tools in immunoassays for chemical contaminants. In this manuscript, nanobodies for the detection of the toxin Nodularin-r (NOD-R), a secondary metabolite of cyanobacteria that could cause a safety risk for drinks and food for its strong hepatotoxicity, were for the first time selected from an immunized Bactrian camel VHH phage display library. Then, a sensitive indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for NOD-R, based on the nanobody N56 with great thermostability and organic solvent tolerance, was established under optimized conditions. The results showed that the limit of detection for NOD-R was 0.67 mu g/L, and the average spike recovery rate was between 84.0 and 118.3%. Moreover, the ic-ELISA method was validated with spiked water sample and confirmed by UPLC-MS/MS, which indicated that the ic-ELISA established in this work is a reproducible detection assay for nodularin residues in water samples.

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