Development and application of KASP marker for high throughput detection of the seedless trait in grapevine

文献类型： 外文期刊

第一作者： Wang, Fu-qiang

作者： Wang, Fu-qiang;Bian, Lu;Qiu, Peng-peng;Guo, Shuo;Guo, Jing-han;Guo, Chen-shuo;Liu, Guo-tian;Wang, Yue-jin;Xu, Yan;Jiang, Jian-fu;Liu, Chong-huai;Wang, Yong

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关键词： seedless grape; MAS; KASP; SSR

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：4.8； 五年影响因子：4.8 ）

ISSN： 2095-3119

年卷期： 2023 年 22 卷 11 期

页码：

收录情况： SCI

摘要： Molecular marker-assisted selection (MAS) can significantly accelerate and improve the efficiency of the breeding process in seedless grape cultivars. In this study, we developed the KASP_VviAGL11 and VviAGL11_410 markers based on a single nucleotide polymorphism (SNP) site (Chr18: 26889437 (A/C)) of the VviAGL11 gene, and compared them with previously reported SSR markers p3_VvAGL11 and 5U_VviAGL11 by testing 101 cultivars and 81 F1 hybrid progenies. The results showed that both of the proposed markers obtained 100% accuracy rates in detecting allele A, which was closely associated with the seedless trait in grapes, while p3_VvAGL11 and 5U_VviAGL11 had lower accuracy rates due to their tendency to produce false positives. After careful evaluation of the technical advantages and disadvantages associated with these markers, we concluded that KASP_VviAGL11 was superior in terms of simplicity, cost-effectiveness, efficiency, and accuracy. Thus, we optimized the process of molecular MAS for seedless grapes, focusing on the KASP_VviAGL11 marker as a central component, to provide key technical support for the development of new seedless grape cultivars.

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