Starch granule-associated proteins of hull-less barley (Hordeum vulgare L.) from the Qinghai-Tibet Plateau in China

文献类型： 外文期刊

第一作者： Wang, Chun-Ping

作者： Wang, Chun-Ping;Pan, Zhi-Fen;Liang, Jun-Jun;Deng, Guang-Bing;Long, Hai;Yu, Mao-Qun;Wang, Chun-Ping;Liang, Jun-Jun;Deng, Guang-Bing;Nima, Zha-Xi;Tang, Ya-Wei;Cai, Peng

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关键词： starch granule-associated proteins; Qinghai-Tibet Plateau; hull-less barley; starch biosynthesis

期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：4.125； 五年影响因子：4.096 ）

ISSN： 0022-5142

年卷期： 2011 年 91 卷 4 期

页码：

收录情况： SCI

摘要： BACKGROUND: The starch granule-associated proteins (SGAPs) are the minor components of the starch granules and a majority of them are believed to be starch biosynthetic enzymes. The Qinghai-Tibet Plateau in China, one of the centres of origin of cultivated barley, is abundant in hull-less barley resources which exhibit high polymorphism in SGAPs. RESULTS: The SGAPs of hull-less barley from Qinghai-Tibet Plateau were analysed by one-dimensional (1-D) SDS-PAGE, 2-D PAGE and ESI-Q-TOF MS/MS. In the 1-D SDS-PAGE gel, four proteins including a 80 kDa starch synthase, actin, actin 4 and ATP synthase beta-subunit were identified as novel SGAPs. A total of six different bands were identified as starch granule-bound starch synthase I (GBSSI) and the segregation of the novel GBSSI bands in F-1 and F-2 seeds derived from yf127 x yf70 was in accordance with Mendel's law. In the 2-D PAGE gel, 92 spots were identified as 42 protein species which could be classified into 15 functional groups. Thirteen protein species were identified as SGAPs for the first time and multiple spots were identified as GBSSI. CONCLUSION: This study revealed novel SGAPs in hull-less barley from the Qinghai-Tibet Plateau in China and these will be significant in further studies of starch biosynthesis in barley. 2011 Society of Chemical Industry

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