Extraction and profiling of proteins in yellow powder from sweet potato starch wastewater using response surface methodology and proteomic approach

文献类型： 外文期刊

第一作者： Yang, Shini

作者： Yang, Shini;Liao, Xiaojun;Yang, Shini;Liu, Haihua;Xu, Zhenzhen;Kong, Xiankui

作者机构：

关键词： Box-Behnken design; protein recovery; proteomics; sweet potato starch; wastewater

期刊名称：JOURNAL OF FOOD SCIENCE （ 影响因子：3.693； 五年影响因子：3.856 ）

ISSN： 0022-1147

年卷期： 2022 年 87 卷 1 期

页码：

收录情况： SCI

摘要： Sweet potato starch industry produce generous high soluble solid wastewater containing various biochemicals such as proteins. The wastewater could be spray dried into a product called yellow powder (YP). Proteins in the YP were recovered and profiled in this study. The extraction conditions were optimized on dependent variables of YP material-water ratio, pH, and temperature using response surface methodology (RSM). Maximum protein yield (61.2%) using RSM were observed at a material-water ratio of 50 (mg/L), pH 9.5, and extraction temperature of 30celcius. Subsequently, a total of 25 proteins were identified by proteomic analysis, which mainly were sporamins, beta-amylase, starch phosphorylase, polyphenol oxidase, and superoxide dismutase. The extraction and profiling of proteins from YP would contribute to a comprehensive utilization and added value of the wastewater produced by sweet potato starch processing industry. Practical Application This study reported the recovery (61.2%) of proteins and protein profile of yellow powder (byproducts) from sweet potato starch wastewater. These information could contribute to the valorization a yellow powder into high-value ingredients.

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