Label-free and TMT-labeled Proteomics Methods to Compare Differences on Normal and Attached Livers of Glyptosternum maculatum

文献类型： 外文期刊

第一作者： Sun, Wu

作者： Sun, Wu;Muhammad, Zafarullah;Guo, Yufeng;Zhang, Guoqiang;Yan, Yingying;Zhang, Guoqiang

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关键词： Glyptosternum maculatum; quantitative proteomic; attached liver; label-free; TMT; comparison of proteomics methods

期刊名称：CURRENT PROTEOMICS （ 影响因子：0.5； 五年影响因子：0.5 ）

ISSN： 1570-1646

年卷期： 2024 年 21 卷 5 期

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收录情况： SCI

摘要： Background The aim of this study is to compare the molecular differences between the normal liver (NG) and attached liver (WG) of Glyptosternum maculatum (G. maculatum) based on label-free and TMT-labeled proteomics data. It provides a theoretical basis for the adaptation of G. maculatum in the plateau area with low temperature and low oxygen. Methods The differentially expressed proteins (DEPs) of label-free and TMT-labeled proteomics data were analyzed by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, and protein structural domain (PSD) enrichment, and protein network interactions were constructed to reveal protein characteristics. Results In the label-free and TMT-labeled groups, we detected a total of 643 and 107 DEPs; 7 common DEPs (co-DEPs) were selected through PPI network information. Higher expression of evm.model.chr5.73, evm.model.chr18.610, evm.model.chr10.1201, and HIF-1 signaling pathway were associated with a hypoxic environment. evm.model.chr15.573, evm.model .chr5.320, evm.model.chr6.360, and evm.model.chr18.610 were associated with WG production. Significant expression of Ubiquitin-like protein 4A and Heat shock 70 kDa protein protects liver cells from injury in a hypothermic and hypoxic environment. Conclusion In terms of the number of DEPs, the labelling-free method is superior to the TMT-labeled method. The TMT-labeled method is better in terms of repeatability and accuracy. The myosin11 not only responds to low temperature and low oxygen but may also lead to WG production through Tight junctions. The NG may be more sensitive than WG in stress response to cancer and viral infections.

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