Transcriptome-Wide Mapping 5-Methylcytosine by m(5)C RNA Immunoprecipitation Followed by Deep Sequencing in Plant

文献类型： 外文期刊

第一作者： Gu, Xiaofeng

作者： Gu, Xiaofeng;Liang, Zhe

作者机构：

关键词： RNA methylation; 5-Methylcytosine; m(5)C; m(5)C-RIP-seq; RNA immunoprecipitation; Plant

期刊名称：PLANT LONG NON-CODING RNAS: Methods and Protocols

ISSN： 1064-3745

年卷期： 2019 年 1933 卷

页码：

收录情况： SCI

摘要： Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m(5)C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m(5)C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.

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