Oxidative stress protective function of ApY(2)SK(2) dehydrin: a late embryogenesis abundant protein in embryogenic callus of Agapanthus praecox to promote post-cryopreservation survival

文献类型： 外文期刊

第一作者： Liu, Tao

作者： Liu, Tao;Zhang, Yiying;Chu, Yunxia;Chen, Hairong;Ren, Li;Liu, Tao;Zhang, Di;Chu, Yunxia

作者机构：

关键词： Dehydrin; Embryogenic callus; Cryopreservation; RNAi; Gene overexpression

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.726； 五年影响因子：2.859 ）

ISSN： 0167-6857

年卷期： 2022 年 149 卷 3 期

页码：

收录情况： SCI

摘要： Dehydrins (DHNs) as the member of the late embryogenesis abundant protein family, play critical roles in seed dehydration protection and plant adaptation to multiple abiotic stresses. As an important method of germplasm preservation, cryopreservation is also an ideal research system to study compound stress. Oxidative stress, as the critical stress in cryopreservation, directly affects cell viability. Our previous in vitro tests indicated that ApY(2)SK(2) DHN can effectively protect enzyme activity and almost double the survival rate of Arabidopsis thaliana seedlings after cryopreservation, but the in vivo protective effect of ApY(2)SK(2) on cryopreservation have not yet been elucidated. In this study, ApY(2)SK(2) type DHN was genetically transformed into embryogenic callus (EC) of Agapanthus praecox by overexpression (OE) and RNA interference (RNAi) techniques to evaluate the in vivo oxidative stress protective effect of DHNs during cryopreservation. The results showed that the cell viability had a completely opposite trend between OE and RNAi cell lines, and the cell relative death ratio of ApY(2)SK(2)-OE EC was significantly decreased 18.5% and ApY(2)SK(2)-RNAi cells was significantly increased 23.5% after cryopreservation. Overexpression ApY(2)SK(2) increased non-enzymatic antioxidant (AsA and GSH) contents, antioxidant enzyme (POD and SOD) activities and up-regulated CAT, POD and GPX expression, while ApY(2)SK(2)-RNAi cells decreased CAT, FeSOD, POD and GPX expression during cryopreservation. These findings suggested that ApY(2)SK(2) can affect ROS metabolism, alleviate H2O2 and OH center dot excessive generation, activate the antioxidant system, improve cellular REDOX balance and reduce membrane lipid peroxidation damage of plant cells during cryopreservation. DHNs can effectively improve cell stress tolerance and have great potential for in vivo or in vitro applications in plant cryopreservation. Key message ApY2SK2 dehydrin can affect ROS metabolism, activate the antioxidant system and reduce membrane lipid peroxidation during cryopreservatin leading to high survival rate after recovery.

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[1]In vivo protective effect of late embryogenesis abundant protein (ApSK(3) dehydrin) on Agapanthus praecox to promote post-cryopreservation survival. Huang, Tingting,Sheng, Jiangyuan,Zhang, Di,Deng, Shan. 2022

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