Ectopic expression of an Old Yellow Enzyme (OYE3) gene from Saccharomyces cerevisiae increases the tolerance and phytoremediation of 2-nitroaniline in rice

文献类型： 外文期刊

第一作者： Li, Zhenjun

作者： Li, Zhenjun;Gao, Jianjie;Wang, Bo;Zhang, Hao;Tian, Yongsheng;Peng, Rihe;Yao, Quanhong

作者机构：

关键词： 2-nitroaniline; Old Yellow Enzyme; Phytoremediation; Reactive oxygen species; Saccharomyces cerevisiae

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2024 年 906 卷

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收录情况： SCI

摘要： 2-nitroaniline (2 -NA) is an environmental pollutant and has been extensively used as intermediates in organic synthesis. The presence of 2 -NA in the environment is not only harmful for aquatic life but also mutagenic for human beings. In this study, we constructed transgenic rice expressing an Old Yellow Enzyme gene, ScOYE3 , from Saccharomyces cerevisiae. The ScOYE3 transgenic plants were comprehensively investigated for their biochemical responses to 2 -NA treatment and their 2 -NA phytoremediation capabilities. Our results showed that the rice seedlings exposed to 2 -NA stress, showed growth inhibition and biomass reduction. However, the transgenic plants exhibited strong tolerance to 2 -NA stress compared to wild -type plants. Ectopic expression of ScOYE3 could effectively protect transgenic plants against 2 -NA damage, which resulted in less reactive oxygen species accumulation in transgenic plants than that in wild -type plants. Our phytoremediation assay revealed that transgenic plants could eliminate more 2 -NA from the medium than wild -type plants. Moreover, omics analysis was performed in order to get a deeper insight into the mechanism of ScOYE3-mediated 2 -NA transformation in rice. Altogether, the function of ScOYE3 during 2 -NA detoxification was characterized for the first time, which serves as strong theoretical support for the phytoremediation potential of 2 -NA by Old Yellow Enzyme genes.

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