Methyltransferase HsdM Regulates the Pathogenicity of Streptococcus agalactiae to Nile Tilapia (Oreochromis niloticus)

文献类型： 外文期刊

第一作者： Jiang, Dongdong

作者： Jiang, Dongdong;Yu, Meiling;Jiang, Dongdong;Wang, Bei;Jiang, Dongdong;Ren, Yan;Mo, Xubing;Zhang, Defeng

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关键词： Streptococcus agalactiae; DNA methyltransferase; adhesion; pathogenesis; growth rate; Nile tilapia

期刊名称：FISHES （ 影响因子：2.4； 五年影响因子：2.4 ）

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年卷期： 2025 年 10 卷 2 期

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收录情况： SCI

摘要： DNA methylation is a critical mechanism for regulating gene expression in bacteria and plays an essential role in bacterial pathogenesis. A mutant, WC1535 Delta hsdM, lacking hsdM encoding a DNA methyltransferase was constructed using homologous recombination technology. The growth, hemolytic activity, and capsule formation of the mutant were analyzed. The dynamic distribution of the wild-type (WT) and mutant strains in tilapia tissues after artificial infection was determined. The adhesion, invasion, anti-phagocytic, and whole-blood survival abilities of the WT and mutant strains were analyzed. Tilapia were intraperitoneally injected with the WT or mutant strains, and the LD50 values were determined. The expression levels of the immune-related genes in tilapia were analyzed by qRT-PCR. The mutant showed faster growth during the logarithmic growth period (5-10 h) and lower hemolytic activity than the WT strain. Mutant loads in tilapia tissues were significantly lower than those of the WT strain. Mutant strain adhesion to epithelial cells was significantly reduced, it was more easily engulfed by macrophages, and it had decreased intracellular survival. The LD50 of the mutant was 2.06 times higher than that of the WT strain, indicating decreased pathogenicity. Expression levels of immune-related genes IL-1 beta, IL-6, IFN-gamma, and TNF-alpha in tilapia induced by the mutant were lower than those by the WT strain. In conclusion, the WC1535 Delta hsdM mutant exhibited an increased growth rate and decreased hemolytic activity, tissue colonization, and pathogenicity, indicating that HsdM could regulate S. agalactiae growth and pathogenicity. This study provides new insights into the pathogenesis of piscine S. agalactiae.

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