Designing and evaluating a novel blood-brain barrier in vitro model of teleost for reproducing alterations in brain infecting

文献类型： 外文期刊

第一作者： Chen, Yanghui

作者： Chen, Yanghui;Li, Yi;Li, Wenze;Huang, Yu;Cai, Jia;Jian, Jichang;Wang, Bei;Chen, Yanghui;Li, Yi;Li, Wenze;Huang, Yu;Cai, Jia;Jian, Jichang;Wang, Bei;Li, Yuan;Zhang, Defeng;Wangkahart, Eakapol

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关键词： Meningitis; Blood-brain barrier; In vitro model; Endothelial cells; Astrocytes

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.9； 五年影响因子：4.2 ）

ISSN： 1050-4648

年卷期： 2025 年 156 卷

页码：

收录情况： SCI

摘要： A new blood-brain barrier (BBB) in vitro model of tilapia ( Oreochromis niloticus) was successfully established by co-culture with brain microvascular endothelial cell line TVEC-01 and brain astrocyte cell line TA-02 of tilapia. Experiments with the expression levels of BBB-related genes, TEER value detection and 4-h water-leaking test have shown that the BBB in vitro model has an excellent barrier effect. In bacterial penetration experiments, the pathogenic strain of Streptococcus agalactiae was able to pass through the BBB in vitro model and initiate a series of immune responses, among which TA-02 contributed to the expression of pro-inflammatory cytokines and TVEC-01 contributed to the expression of anti-inflammatory cytokines, providing an excellent research tool and theoretical basis for further study of meningitis. Moreover, the qRT-PCR and transmission electron microscopy revealed that the pathogenic strain of S. agalactiae effectively penetrated the BBB in vitro model of tilapia during early-stage infection without destroying tight junction integrity. This suggested that, in the initial phases of infection, the pathogenic strain of S. agalactiae may breach the BBB via a transcellular pathway rather than a paracellular pathway. Summarily, a novel BBB in vitro model of tilapia was successfully designed and evaluated for reproducing alterations in brain infecting.

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