Characterization of a barley (Hordeum vulgare L.) mutant with multiple stem nodes and spikes and dwarf (msnsd) and fine-mapping of its causal gene

文献类型： 外文期刊

第一作者： Qin, Dandan D.

作者： Qin, Dandan D.;Liu, Rui;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Cheng, Hongna;Dong, Jing;Qin, Dandan D.;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Dong, Jing;Qin, Dandan D.;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Dong, Jing;Liu, Rui;Dong, Guoqing;Xu, Le;Cheng, Hongna;Guo, Ganggang;Li, Chengdao

作者机构：

关键词： barley; BSR-seq; TCP; multiple stem nodes and spikes and dwarf; fine-mapping

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： IntroductionMultiple nodes and dwarf mutants in barley are a valuable resource for identifying genes that control shoot branching, vegetative growth and development. MethodsIn this study, physiological, microscopic and genetic analysis were conducted to characterize and fine-map the underling gene of a barley mutant with Multiple Stem Nodes and Spikes and Dwarf (msnsd), which was selected from EMS- and Co-60-treated barley cv. Edamai 934. Results and discussionThe msnsd mutant had more stem nodes, lower plant height and a shorter plastochron than Edamai 934. Moreover, the mutant had two or more spikes on each tiller. Microscopic analysis showed that the dwarf phenotype of msnsd resulted from reduced cell lengths and cell numbers in the stem. Further physiological analysis showed that msnsd was GA(3)-deficient, with its plant height increasing after external GA(3) application. Genetic analysis revealed that a single recessive nuclear gene, namely, HvMSNSD, controlled the msnsd phenotype. Using a segregating population derived from Harrington and the msnsd mutant, HvMSNSD was fine-mapped on chromosome 5H in a 200 kb interval using bulked segregant analysis (BSA) coupled with RNA-sequencing (BSR-seq), with a C-T substitution in the exon of HvTCP25 co-segregating with the msnsd phenotype. RNA-seq analysis showed that a gene encoding gibberellin 2-oxidase 8, a negative regulator of GA biosynthesis, was upregulated in the msnsd mutant. Several known genes related to inflorescence development that were also upregulated and enriched in the msnsd mutant. Collectively, we propose that HvMSNSD regulates the plastochron and morphology of reproductive organs, likely by coordinating GA homeostasis and changed expression of floral development related genes in barley. This study offers valuable insights into the molecular regulation of barley plant architecture and inflorescence development.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序