Membrane Protein Bcest Is Involved in Hyphal Growth, Virulence and Stress Tolerance of Botrytis cinerea

文献类型： 外文期刊

第一作者： Zhang, Wei

作者： Zhang, Wei;Ge, Bei-Bei;Lv, Zhao-Yang;Shi, Li-Ming;Zhang, Ke-Cheng;Park, Kyung Seok

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关键词： Bcest; Botrytis cinerea; germination; hyphal growth; pathogenicity; stress tolerance

期刊名称：MICROORGANISMS （ 影响因子：4.5； 五年影响因子：4.8 ）

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年卷期： 2023 年 11 卷 5 期

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收录情况： SCI

摘要： Botrytis cinerea is a necrotrophic model fungal plant pathogen that causes grey mould, a devastating disease responsible for large losses in the agriculture sector. As important targets of fungicides, membrane proteins are hot spots in the research and development of fungicide products. We previously found that membrane protein Bcest may be closely related to the pathogenicity of Botrytis cinerea. Herein, we further explored its function. We generated and characterised ?Bcest deletion mutants of B. cinerea and constructed complemented strains. The ?Bcest deletion mutants exhibited reduced conidia germination and germ tube elongation. The functional activity of ?Bcest deletion mutants was investigated by reduced necrotic colonisation of B. cinerea on grapevine fruits and leaves. Targeted deletion of Bcest also blocked several phenotypic defects in aspects of mycelial growth, conidiation and virulence. All phenotypic defects were restored by targeted-gene complementation. The role of Bcest in pathogenicity was also supported by reverse-transcriptase real-time quantitative PCR results indicating that melanin synthesis gene Bcpks13 and virulence factor Bccdc14 were significantly downregulated in the early infection stage of the ?Bcest strain. Taken together, these results suggest that Bcest plays important roles in the regulation of various cellular processes in B. cinerea.

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