Direct isolation of high-quality low molecular weight RNA of pear peel from the extraction mixture containing nucleic acid

文献类型： 外文期刊

第一作者： Wang, Xin-wei

作者： Wang, Xin-wei;Zhang, Zhen;Qiao, Yu-shan;Xiong, Ai-sheng;Yao, Quan-hong

作者机构：

关键词： Low molecular weight RNA (LMW RNA);MicroRNAs;Pear peel;Polyethylene glycol (PEG) precipitation

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Low molecular weight RNA (LMW RNA) is generally obtained either from the total RNA or from total nucleic acids solution. Many steps and chemical reagents are involved in traditional methods for LMW RNA isolation where degradation of LMW RNA often occurs, especially for plant materials with high levels of secondary catabolites. In this study, an efficient method was developed to directly isolate pure LMW RNA from pear peel, a material rich in polyphenolics that is covered with a layer of wax. The method was based on polyethylene glycol (PEG) precipitation combining CTAB buffer which is often used to isolate RNA from polysaccharide-rich and polyphenolics-rich materials. The entire procedure could be completed within 6 h and many samples could be processed at the same time. Few and common chemicals are used with this method. Hence, it could be used as an ordinary method in the laboratory. The developed method was further tested by isolating LMW RNA from Arabidopsis. Using the isolated LMW RNA samples, microRNAs were successfully detected and characterized.

分类号： Q7

  • 相关文献

[1]Multiple promoters and targeted microRNAs direct the expressions of HMGB3 gene transcripts in dairy cattle. Li, Liming,Huang, Jinming,Ju, Zhihua,Li, Qiuling,Wang, Changfa,Qi, Chao,Zhang, Yan,Hou, Qinlei,Zhong, Jifeng,Li, Liming,Hang, Suqin. 2013

[2]Dimeric artificial microRNAs mediate high resistance to RSV and RBSDV in transgenic rice plants. Sun, Lin,Lin, Chao,Du, Jinwen,Song, Yunzhi,Liu, Hongmei,Zhou, Shumei,Wen, Fujiang,Zhu, Changxiang,Jiang, Mingsong.

[3]Comparative Analysis of Cotton Small RNAs and Their Target Genes in Response to Salt Stress. Zujun Yin ,Fu, Xiaoqiong,Ye, Wuwei,Xiulan Han,Yan Li,Junjuan Wang,Delong Wang,Shuai Wang,Xiaoqiong Fu,Wuwei Ye. 2017

[4]Identification of MicroRNAs in Wild Soybean (Glycine soja). Chen, Rui,Hu, Zheng,Zhang, Hui. 2009

[5]Altered levels of circulating miRNAs are associated Schistosoma japonicum infection in mice. Zhu, Lihui,Dao, Jinwei,Du, Xiaoli,Li, Hao,Lu, Ke,Liu, Jinming,Cheng, Guofeng. 2015

[6]Identification and characterization of lymph organ microRNAs in red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus. Du, Zhi-Qiang,Leng, Xiao-Yun,Jin, Yan-Hui,Shen, Xiu-Li,Li, Xin-Cang. 2017

[7]Role of microRNAs in aluminum stress in plants. He, Longfei,Gu, Minghua,He, Huyi. 2014

[8]tRNA Derived smallRNAs: smallRNAs Repertoire Has Yet to Be Decoded in Plants. Sablok, Gaurav,Sablok, Gaurav,Yang, Kun,Wen, Xiaopeng,Yang, Kun,Wen, Xiaopeng,Chen, Rui. 2017

[9]Solexa Sequencing of MicroRNAs on Chromium Metabolism in Broiler Chicks. Pan, Y. Z.,Wu, S. G.,Zhang, H. J.,Yue, H. Y.,Qi, G. H.,Pan, Y. Z.,Dai, H. C.. 2013

[10]The discovery approaches and detection methods of microRNAs. Huang, Yong,Wang, Sheng Peng,Tang, Shun Ming,Zhang, Guo Zheng,Shen, Xing Jia,Huang, Yong,Wang, Sheng Peng,Tang, Shun Ming,Zhang, Guo Zheng,Shen, Xing Jia,Zou, Quan. 2011

[11]Parsing the Regulatory Network between Small RNAs and Target Genesin Ethylene Pathway in Tomato. Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Wang, Yunxiang,Wang, Qing,Gao, Lipu,Zuo, Jinhua,Zhu, Benzhong,Ju, Zheng,Luo, Yunbo. 2017

[12]Identification of Differentially Expressed Micrornas Associate with Glucose Metabolism in Different Organs of Blunt Snout Bream (Megalobrama amblycephala). Miao, Ling-Hong,Pan, Wen-Jing,Huang, Xin,Ge, Xian-Ping,Liu, Bo,Miao, Ling-Hong,Lin, Yan,Ge, Xian-Ping,Ren, Ming-Chun,Zhou, Qun-Lan,Liu, Bo. 2017

[13]IDENTIFICATION OF miRNAs IN SWEET POTATO BY SOLEXA SEQUENCING. Bian, X.,Ma, P.,Jia, Z.,Guo, X.,Xie, Y.,E, Z..

[14]Expression of a vitelline membrane protein, BmVMP23, is repressed by bmo-miR-1a-3p in silkworm, Bombyx mori. Zhao, Qiaoling.

[15]Systematic discovery and characterization of stress-related microRNA genes in Oryza sativa. Xie, Kai Bin,Zhou, Xue,Zhang, Tian Hai,Chen, Guo Xiang,Zhou, Xue,Zhang, Bao Long,Chen, Li Ming.

[16]Genome-Wide Identification of Novel microRNAs from Genome Sequences Using Computational Approach in the Mudskipper (Boleophthalmus pectinirostris). Gong, Wangbao,Xie, Jun,Wang, Guangjun,Yu, Deguang,Huang, Yong,Sun, Xihong.

[17]MicroRNAs Involved in Skeletal Muscle Differentiation. Zhang, Xiquan. 2013

[18]Epigenetics in Schistosomes: What We know and What We Need know. Liu, Weiwei. 2016

[19]Identification of novel microRNAs in the Verticillium wilt-resistant upland cotton variety KV-1 by high-throughput sequencing. Xiaohong He,Quan Sun,Huaizhong Jian,Xiaoyan Zhu,Jianchuan Mo,Lu Long,Liuxin Xiang,Yongfang Xie,Yuzhen Shi,Youlu Yuan,Yingfan Cai. 2014

[20]MicroRNAs can effectively induce formation of insulin-producing cells from mesenchymal stem cells. Bai, Chunyu,Gao, Yuhua,Li, Xiangchen,Wang, Kunfu,Xiong, Hui,Shan, Zhiqiang,Zhang, Ping,Wang, Wenjie,Guan, Weijun,Ma, Yuehui. 2017

作者其他论文 更多>>

微信小程序