Overexpression of auxin synthesis gene PagYUC6a in poplar (Populus alba P. glandulosa) enhances salt tolerance

文献类型： 外文期刊

第一作者： Zhao, Yanqiu

作者： Zhao, Yanqiu;Song, Shuo;Guo, Yu;Tian, Zhaoyang;Shang, Yuanyuan;Ding, Yihao;Li, Xiaoyu;Zhao, Lizi;Zhang, Hongxia;Zhao, Yanqiu;Song, Shuo;Guo, Yu;Tian, Zhaoyang;Shang, Yuanyuan;Ding, Yihao;Li, Xiaoyu;Zhao, Lizi;Zhang, Hongxia;Zhang, Hongxia;Zhang, Hongxia;Zhang, Hongxia

作者机构：

关键词： PagYUC6a; Transgenic poplar; Salt response; ROS scavenging

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 311 卷

页码：

收录情况： SCI

摘要： YUCCA proteins play a crucial role in auxin biosynthesis. However, their specific functions in poplar in response to abiotic stress are unclear. Here, we isolated the auxin biosynthesis gene PagYUC6a, one of the Arabidopsis AtYUC6 homologs, from '84 K' poplar (Populus alba x P. glandulosa), and investigated its role in salt tolerance in transgenic poplar plants. PagYUC6a was predominantly expressed in young stems and significantly upregulated upon 200 mM NaCl treatment. Overexpression of PagYUC6a enhanced the growth and salt tolerance of trans-genic poplar, as well as the content of indole-3-acetic acid (IAA). Further analysis revealed that the level of reactive oxygen species (ROS) accumulation in the leaves of transgenic plants was significantly lower than that in the leaves of wild type plants, accompanied with a higher activity of catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX). The transcript level of CAT2, SOD1 and APX1 was also higher in the leaves of transgenic plants under salt stress conditions. These results demonstrated that PagYUC6a improved salt tolerance by enhancing ROS scavenging capacity. Our findings provide valuable information on the molecular mechanisms of PagYUC6a mediated salinity tolerance and highlight its potential for the breeding of salt-tolerant tree species.

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