Calcineurin B-like interacting protein kinase 31 confers resistance to sheath blight via modulation of ROS homeostasis in rice

文献类型： 外文期刊

第一作者： Chen, Huan

作者： Chen, Huan;Lin, Qiujun;Li, Zhuo;Mei, Qiong;Xuan, Yuanhu;Lin, Qiujun;Chu, Jin;Dong, Hai;Mei, Qiong;Xuan, Yuanhu

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关键词： CIPK31; homeostasis; resistance; rice; ROS; sheath blight

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.9 ）

ISSN： 1464-6722

年卷期： 2023 年 24 卷 3 期

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收录情况： SCI

摘要： Sheath blight (ShB) severely threatens rice cultivation and production; however, the molecular mechanism of rice defence against ShB remains unclear. Screening of transposon Ds insertion mutants identified that Calcineurin B-like protein-interacting protein kinase 31 (CIPK31) mutants were more susceptible to ShB, while CIPK31 overexpressors (OX) were less susceptible. Sequence analysis indicated two haplotypes of CIPK31: Hap_1, with significantly higher CIPK31 expression, was less sensitive to ShB than the Hap_2 lines. Further analyses showed that the NAF domain of CIPK31 interacted with the EF-hand motif of respiratory burst oxidase homologue (RBOHA) to inhibit RBOHA-induced H2O2 production, and RBOHA RNAi plants were more susceptible to ShB. These data suggested that the CIPK31-mediated increase in resistance is not associated with RBOHA. Interestingly, the study also found that CIPK31 interacted with catalase C (CatC); cipk31 mutants accumulated less H2O2 while CIPK31 OX accumulated more H2O2 compared to the wild-type control. Further analysis showed the interaction of the catalase domain of CatC with the NAF domain of CIPK31 by which CIPK31 inhibits CatC activity to accumulate more H2O2.

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