Coupling of gene regulation and carrier modification manipulates bacterial biofilms as robust living catalysts

文献类型： 外文期刊

第一作者： Yan, Cheng-Hai

作者： Yan, Cheng-Hai;Zhan, Yu-Fan;Chen, Huan;Herman, Richard A.;Xu, Yan;Khurshid, Marriam;Gong, Lu-Chan;You, Shuai;Wang, Jun;Xu, Yan;Gong, Lu-Chan;You, Shuai;Wang, Jun;Wang, Jun

作者机构：

关键词： Biofilm; Living catalysts; C sg D; Gene overexpression; Carrier modification

期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）

ISSN： 0960-8524

年卷期： 2024 年 399 卷

页码：

收录情况： SCI

摘要： The biofilm of an engineered strain is limited by slow growth and low yield, resulting in an unsatisfactory ability to resist external stress and promote catalytic efficiency. Here, biofilms used as robust living catalysts were manipulated through dual functionalized gene regulation and carrier modification strategies. The results showed that gene overexpression regulates the autoinducer-2 activity, extracellular polymeric substance content and colony behavior of Escherichia coli , and the biofilm yield of csg D overexpressed strains increased by 79.35 % compared to that of the wild type strains ( p < 0.05). In addition, the hydrophilicity of polyurethane fibres modified with potassium dichromate increased significantly, and biofilm adhesion increased by 105.80 %. Finally, the isoquercitrin yield in the catalytic reaction of the biofilm reinforced by the csg D overexpression strain and the modified carrier was 247.85 % higher than that of the untreated group. Overall, this study has developed engineered strains biofilm with special functions, providing possibilities for catalytic applications.

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