An epi-allele of SMS causes Sanming dominant genic male sterility in rice

文献类型： 外文期刊

第一作者： Yang, Yachun

作者： Yang, Yachun;Li, Hao;Xu, Zuntao;Tai, Dewei;Ni, Dahu;Wei, Pengcheng;Yi, Chengxin;Yang, Jianbo;Yang, Yachun;Zhang, Cheng;Li, Hao;Xu, Zuntao;Tai, Dewei;Ding, Yong;Zhang, Cheng;Yang, Zeyuan;Xu, Zuntao;Ding, Yong

作者机构：

关键词： male sterility; rice; epi-allele; Sanming; DNA methylation

期刊名称：SCIENCE CHINA-LIFE SCIENCES （ 影响因子：9.1； 五年影响因子：7.1 ）

ISSN： 1674-7305

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Male sterility is an important trait in rice for hybrid rice (Oryza sativa) breeding. However, the factors involved in dominant male sterility are largely unknown. Here, we identified a gene from Sanming dominant genic male sterile rice, named Sanming dominant male sterility (SMS), and reported that an epi-allele of this locus contributes to male sterility. Segregation analysis attributed dominant male sterility to a single locus, SMS, which we characterized using a male-sterile near isogenic line (NIL) of rice cultivar 93-11. The SMS locus was heterozygous in the male-sterile 93-11 NIL, containing an epi-allele identical to that in 93-11, and an epi-allele identical to that in rice cultivar Nipponbare, which we refer to as SMS9 and SMSN, respectively. SMS9 is silent and hyper-methylated, whereas SMSN is expressed and hypo-methylated in the 93-11 NIL. Overexpressing SMSN led to male sterility. Mutations in SMS rescued the male sterility of the 93-11 NIL. Interestingly, we observed the duplication of SMSN in Nipponbare, but did not observe the duplication of SMS9 in 93-11. Together, these findings suggest that the reduced methylation and enhanced expression of the SMSN epi-allele in the 93-11 NIL is responsible for its role in conferring dominant male sterility.

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