Characterization, polymorphism assessment, and database construction for microsatellites from BAC end sequences of channel catfish (Ictalurus punctatus): a resource for integration of linkage and physical maps

文献类型： 外文期刊

第一作者： Somridhivej, Benjapom

作者： Somridhivej, Benjapom;Wang, Shaolin;Sha, Zhenxia;Liu, Hong;Quilang, Jonas;Xu, Peng;Li, Ping;Liu, Zhanjiang;Sha, Zhenxia;Liu, Hong;Quilang, Jonas;Hue, Zhiliang

作者机构：

关键词： bacterial artificial chromosomes;gene mapping;genetic markers;genetic polymorphism;genome analysis;genomes;linkage;microsatellites;nucleotide sequences;polymerase chain reaction;Ictalurus punctatus

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： To apply genome-based technologies for genetic improvements using marker-assisted selection, genome research involving genetic linkage mapping and physical mapping is required, and integration of genetic and linkage maps would significantly enhance the capacities for genome research. In catfish, the major aquaculture species in the United States, linkage and physical maps have been constructed. However, integration of genetic linkage and physical maps demands large-scale, genome-wide hybridizations, or genetic mapping of polymorphic markers derived from bacterial artificial chromosome (BAC) clones whose locations are known from the physical map. In this work, we identified a large number of microsatellites from BAC end sequences of channel catfish, characterized the microsatellites, tested their utility for linkage mapping in a resource family used for genetic mapping, and constructed a web-searchable database for BAC end sequences, their linked microsatellites, microsatellite primers, PCR conditions, and polymorphic information. A total of 3652 microsatellites in 2744 distinct BACs were identified. Of these, 1100 had sufficient and complex flanking sequences for PCR primer design. We have tested 500 primer pairs and found 211 (42.2%) were polymorphic and segregating in the resource family used for genetic mapping. These microsatellites represent a major fraction of co-dominant polymorphic markers identified to date in catfish, and should be a valuable resource for genetic mapping to increase linkage map resolution, and for integration of genetic linkage and physical maps.

分类号： S9

  • 相关文献

[1]Identification and validation of a core set of microsatellite markers for genetic diversity analysis in watermelon, Citrullus lanatus Thunb. Matsum. & Nakai. Zhang, Haiying,Wang, Hui,Guo, Shaogui,Ren, Yi,Gong, Guoyi,Xu, Yong,Weng, Yiqun.

[2]Construction of a Genetic Linkage Map and Mapping of a Female-Specific DNA Marker in Half-Smooth Tongue Sole (Cynoglossus semilaevis). Liao, Xiaolin,Ma, Hong-Yu,Xu, Gen-Bo,Shao, Chang-Wei,Tian, Yong-Sheng,Ji, Xiang-Shan,Yang, Jing-Feng,Chen, Song-Lin.

[3]QTL mapping for fiber quality traits across multiple generations and environments in upland cotton. Fu-Ding Sun,Jian-Hong Zhang,Shu-Fang Wang,Wan-Kui Gong,Yu-Zhen Shi,Ai-Ying Liu,Jun-Wen Li,Ju-Wu Gong,Hai-Hong Shang,You-Lu Yuan.

[4]Genetic diversity among a founder parent and widely grown wheat cultivars derived from the same origin based on morphological traits and microsatellite markers. Li, X. J.,Xu, X.,Yang, X. M.,Li, X. Q.,Liu, W. H.,Gao, A. N.,Li, L. H.,Li, X. J.,Xu, X..

[5]SSR marker-based mapping and linkage analysis of Bombyx mori thermotolerance gene. Zhao, Yuan,Wu, Yang-Chun,Zhu, Ya-Hong,Zhao, Yuan,Zhang, Jiang.

[6]Comparative LD mapping using single SNPs and haplotypes identifies QTL for plant height and biomass as secondary traits of drought tolerance in maize. Lu, Yanli,Xu, Jie,Yuan, Zhimin,Lan, Hai,Rong, Tingzhao,Lu, Yanli,Xu, Yunbi,Xu, Yunbi,Shah, Trushar.

[7]Mapping of clubroot (Plasmodiophora brassicae) resistance in canola (Brassica napus). Zhang, H.,Sun, R.,Zhang, H.,Strelkov, S. E.,Feng, J.,Hwang, S. -F.,Falak, I.,Huang, X..

[8]Genetic and cytological analysis of a new spontaneous male sterility in radish (Raphanus sativus L.). Wang, Zhi-Wei,Gao, Lei,Zhou, Yuan,Wang, Ting,Wang, Zhi-Wei,Xiang, Chang Ping,Liu, Hai Zhou,Mei, Shi Yong.

[9]Functional markers in wheat: current status and future prospects. Liu, Yanan,He, Zhonghu,Xia, Xianchun,He, Zhonghu,Appels, Rudi.

[10]Phylogenetic relationships of wild roses in China based on nrDNA and matK data. Qiu, Xianqin,Zhang, Hao,Wang, Qigang,Jian, Hongying,Yan, Huijun,Zhang, Ting,Wang, Jihua,Tang, Kaixue.

[11]Meat quality and carcass traits in relation to HGD-BstXI and HGD-HaeIII PCR-RFLP polymorphism in Chinese red cattle. Cao, Y.,Zhang, L. C.,Yu, Y. S.,Jin, H. G.,Zhou, G. L.,Zhou, G. L.,Li, M..

[12]Use of mitochondrial RNA genes for the differentiation of four Trichinella species by multiplex PCR amplification. Blaga, R.,Fu, BaoQuan,Le Rhun, D.,Le Naour, E.,Heckman, A.,Zocevic, A.,Boireau, P.,Liu, MingYuan.

[13]Screening of strain-specific Actinobacillus pleuropneumoniae genes using a combination method. Lei, Liancheng,Wang, Jiaqi,Lei, Liancheng,Du, Chongtiao,Yang, Peng,Xie, Fang,Ou, Pingyang,Han, Wenyu.

[14]Molecular cloning and characterization of Bombyx mori CREB gene. Song, Hongsheng,Sun, Yue,Zhang, Yang,Li, Muwang.

[15]Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.). Wang, Linhai,Xia, Xianchun,He, Zhonghu,Li, Genying,Pena, Roberto J.,He, Zhonghu.

[16]Development of microsatellite markers for southern catfish (Silurus meriaionalis) and cross-species amplification in northern sheatfish (Silurus soldatovi). Quan, Y. C.,Sun, X. W.,Liang, L. Q.. 2007

[17]Genetic diversity within Oryza rufipogon germplasms preserved in Chinese field gene banks of wild rice as revealed by microsatellite markers. Zhang, Chi-Hong,Li, Dao-Yuan,Pan, Da-Jian,Jia, Ji-Zeng,Dong, Yu-Shen.

[18]A novel genome of C and the first autotetraploid species in the Setaria genus identified by genomic in situ hybridization. Wang, Yongqiang,Zhi, Hui,Li, Wei,Li, Haiquan,Wang, Yongfang,Diao, Xianmin,Wang, Yongfang,Huang, Zhanjing,Diao, Xianmin,Zhi, Hui,Diao, Xianmin.

[19]Genomic in situ hybridization (GISH) discriminates between the A and the B genomes in diploid and tetraploid Setaria species. Benabdelmouna, A,Shi, Y,Abirached-Darmency, M,Darmency, H. 2001

[20]Isolation and Characterization of Lytic Phage vB_EcoM_JS09 against Clinically Isolated Antibiotic-Resistant Avian Pathogenic Escherichia coli and Enterotoxigenic Escherichia coli. Zhou, Yan,Bao, Hongduo,Zhang, Hui,Wang, Ran.

作者其他论文 更多>>

微信小程序