Transcriptomic analysis of hub genes regulating albinism in light- and temperature-sensitive albino tea cultivars 'Zhonghuang 1' and 'Zhonghuang 2'

文献类型： 外文期刊

第一作者： Wang, Lu

作者： Wang, Lu;Di, Taimei;Li, Nana;Peng, Jing;Wu, Yedie;He, Mingming;Hao, Xinyuan;Huang, Jianyan;Ding, Changqing;Yang, Yajun;Wang, Xinchao

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关键词： Albino; Chloroplast; Tea plant (Camellia sinensis); Transcriptomic analysis

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：5.1； 五年影响因子：5.3 ）

ISSN： 0167-4412

年卷期： 2024 年 114 卷 3 期

页码：

收录情况： SCI

摘要： Albino tea cultivars have high economic value because their young leaves contain enhanced free amino acids that improve the quality and properties of tea. Zhonghuang 1 (ZH1) and Zhonghuang 2 (ZH2) are two such cultivars widely planted in China; however, the environmental factors and molecular mechanisms regulating their yellow-leaf phenotype remain unclear. In this study, we demonstrated that both ZH1 and ZH2 are light- and temperature-sensitive. Under natural sunlight and low-temperature conditions, their young shoots were yellow with decreased chlorophyll and an abnormal chloroplast ultrastructure. Conversely, young shoots were green with increased chlorophyll and a normal chloroplast ultrastructure under shading and high-temperature conditions. RNA-seq analysis was performed for high light and low light conditions, and pairwise comparisons identified genes exhibiting different light responses between albino and green-leaf cultivars, including transcription factors, cytochrome P450 genes, and heat shock proteins. Weighted gene coexpression network analyses of RNA-seq data identified the modules related to chlorophyll differences between cultivars. Genes involved in chloroplast biogenesis and development, light signaling, and JA biosynthesis and signaling were typically downregulated in albino cultivars, accompanied by a decrease in JA-ILE content in ZH2 during the albino period. Furthermore, we identified the hub genes that may regulate the yellow-leaf phenotype of ZH1 and ZH2, including CsGDC1, CsALB4, CsGUN4, and a TPR gene (TEA010575.1), which were related to chloroplast biogenesis. This study provides new insights into the molecular mechanisms underlying leaf color formation in albino tea cultivars. ZH1 and ZH2 were identified as light- and temperature-sensitive albino tea cultivars. WGCNAidentified hub genes regulating the albino phenotype of ZH1 and ZH2.

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