Nuclease Triggered "Signal-On" and Amplified Fluorescent Sensing of Fumonisin B-1 Incorporating Graphene Oxide and Specific Aptamer

文献类型： 外文期刊

第一作者： Guo, Xiaodong

作者： Guo, Xiaodong;Zhang, Mengke;Guo, Xiaodong;Fauconnier, Marie-Laure;Guo, Xiaodong;Qiao, Qinqin;Qiao, Qinqin

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关键词： aptasensor; fumonisin B-1; nuclease; graphene oxide; point-of-care testing; food safety

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 16 期

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收录情况： SCI

摘要： Remarkable advancements have been achieved in the development of rapid analytic techniques toward fumonisin B-1 (FB1) monitoring and even trace levels for food safety in recent years. However, the point-of-care testing for quantitative and accurate FB1 determination is still challenging. Herein, an innovative aptasensor was established to monitor FB1 by utilizing graphene oxide (GO) and nuclease-triggered signal enhancement. GO can be utilized as a fluorescence quenching agent toward a fluorophore-modified aptamer, and even as a protectant of the aptamer from nuclease cleavage for subsequent target cycling and signal amplification detection. This proposed sensing strategy exhibited a good linearity for FB1 determination in the dynamic range from 0.5 to 20 ng mL(-1) with a good correlation of R-2 = 0.995. Its limit of detection was established at 0.15 ng mL(-1) (S/N = 3), which was significantly lower than the legal requirements by three orders of magnitude. The interferent study demonstrated that the introduced aptasensor possessed high selectivity for FB1. Moreover, the aptasensor was successfully applied to the detection of wheat flour samples, and the results were consistent with the classical ELISA method. The rapid response, sensitive and selective analysis, and reliable results of this sensing platform offer a promising opportunity for food mycotoxin control in point-of-care testing.

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