Prevalence and Evolutionary Characteristics of Bovine Coronavirus in China

文献类型： 外文期刊

第一作者： Li, Siyuan

作者： Li, Siyuan;Huang, Jin;Cai, Xuhang;Mao, Li;Yuan, Xuesong;Sun, Xinru;Fan, Baochao;Li, Jizong;Li, Bin;Li, Siyuan;Cai, Xuhang;Mao, Li;Yuan, Xuesong;Sun, Xinru;Li, Jizong;Li, Bin;Li, Siyuan;Cai, Xuhang;Xu, Xingang;Huang, Jin;Yuan, Xuesong;Fan, Baochao;Li, Jizong;Li, Bin;Mao, Li;Fan, Baochao;Li, Jizong;Li, Bin;Mao, Li;Fan, Baochao;Li, Jizong;Li, Bin;Xie, Lingling;Wang, Fu;Zhou, Hua;Fu, Xincheng

作者机构：

关键词： bovine coronavirus; cattle; diarrhea; genotypes; genetic evolution

期刊名称：VETERINARY SCIENCES （ 影响因子：2.0； 五年影响因子：2.2 ）

ISSN：

年卷期： 2024 年 11 卷 6 期

页码：

收录情况： SCI

摘要： Simple Summary Bovine coronavirus (BCoV), bovine rotavirus, bovine viral diarrhea virus, and bovine astrovirus are the most common intestinal pathogenic viruses causing diarrhea in cattle. BCoV was the major pathogen detected, with a positive rate of 34.02% (560/1646) from January 2020 to August 2023. The asymptomatic BCoV-infected rate (351/1646) was higher than the diarrhea BCoV-infected rate (209/1646). Studying the relevance of diarrhea associated with BCoV has shown that the onset of diarrheal symptoms post-infection is strongly correlated with the cattle's age and may also be related to the breed. One the other hand, we amplified and sequenced the hemagglutinin esterase (HE), spike protein, and whole genomes of the partially positive samples and obtained six complete HE sequences, seven complete spike sequences, and six whole genomes. The results of molecular characterization revealed that six strains were in the GIIb subgroup, and HBSJZ2202 and JSYZ2209 had four amino acid insertions on HE. We also analyzed ORF1a and found disparities across various regions within GIIb, which were positioned on separate branches within the phylogenetic tree. This work provides data for further investigating the epidemiology of BCoV and for understanding and analyzing BCoV distribution and dynamics. Bovine coronavirus (BCoV), bovine rotavirus, bovine viral diarrhea virus, and bovine astrovirus are the most common intestinal pathogenic viruses causing diarrhea in cattle. We collected 1646 bovine fecal samples from January 2020 to August 2023. BCoV was the major pathogen detected, with a positive rate of 34.02% (560/1646). Of the 670 diarrheal samples and 976 asymptomatic samples, 209 and 351 were BCoV-positive, respectively. Studying the relevance of diarrhea associated with BCoV has shown that the onset of diarrheal symptoms post-infection is strongly correlated with the cattle's age and may also be related to the breed. We amplified and sequenced the hemagglutinin esterase (HE), spike protein, and whole genomes of the partially positive samples and obtained six complete HE sequences, seven complete spike sequences, and six whole genomes. Molecular characterization revealed that six strains were branched Chinese strains, Japanese strains, and partial American strains from the GIIb subgroup. Strains HBSJZ2202 and JSYZ2209 had four amino acid insertions on HE. We also analyzed ORF1a and found disparities across various regions within GIIb, which were positioned on separate branches within the phylogenetic tree. This work provides data for further investigating the epidemiology of BCoV and for understanding and analyzing BCoV distribution and dynamics.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序