DNA Methylome Provide New Insights into the Physiological-Molecular Regulation of Salt Stress in Kenaf Using 5-azaC Pretreatment

文献类型： 外文期刊

第一作者： Li, Zengqiang

作者： Li, Zengqiang;Luo, Dengjie;Cao, Shan;Mubeen, Samavia;Rehman, Muzammal;Wang, Caijin;Chen, Peng;Li, Zengqiang;Luo, Dengjie;Li, Ru;Jin, Gang;Chen, Tao

作者机构：

关键词： NaCl; Saline stress; Hibiscus cannabinus; Epigenetics; 5-azacytidine (5-azaC); HcROS1

期刊名称：JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION （ 影响因子：3.9； 五年影响因子：3.9 ）

ISSN： 0718-9508

年卷期： 2024 年

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收录情况： SCI

摘要： Salinity affects plant growth, alters physiology and causes changes in DNA methylation. However, complete understanding of DNA methylation mechanism in response to salt stress remains unclear. Kenaf seedlings were pre-treated with 0, 50, 100, 150, and 200 mu M of the DNA methylation inhibitor 5-azacytidine (5-azaC) for 7 days, and exposed to uniform NaCl concentration (150 mM) in nutrient solution for 9 days. Morpho-physiological, hormonal, and ultrastructural changes of leaves and DNA methylation sequencing were investigated, and function of DNA demethylase gene HcROS1 was verified by virusinduced gene silencing (VIGS) technology. Pretreatment with 5-azaC alleviated salt stress in kenaf, significantly increased the seedlings biomass, antioxidant enzyme activities, and contents of chlorophyll and carbohydrates while, reduced ROS production. Furthermore, 150 mu M 5-azaC pretreatment relieved salt stress damage to cell ultrastructure, particularly chloroplast structure, whose lamellar structure was neatly stacked. Methylome analysis showed that 5-azaC pretreatment significantly reduced genomic DNA methylation, and a total of 441 differentially methylated genes (DMGs) were detected in 5-azaC150, of which 186 and 255 were characterized as up- and down- regulated DMGs, respectively. DMGs HcMDH, pyruvate kinase, triosephosphate isomerase, G6PDH, NADPH, and Hsps are mainly involved in carbon metabolism, amino acid biosynthesis, and fatty acid metabolism were characterized as differentially expressed genes (DEGs). Moreover, silencing HcROS1 could significantly increased the sensitivity of kenaf seedlings to salt. 5-azaC pretreatment altered physiological indexes, reduced DNA methylation levels, and improved kenaf salt tolerance. Furthermore, HcROS1 can positively regulate kenaf's response to salt stress.

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