Exogenous nitric oxide alleviates cadmium toxicity in kenaf ( Hibiscus cannabinus L.) through modulating Cd deposition and regulating key genes and involved pathways

文献类型： 外文期刊

第一作者： Cao, Shan

作者： Cao, Shan;Pan, Jiao;Rehman, Muzammal;Luo, Dengjie;Wang, Qiuping;Chen, Peng;Li, Ru;Jin, Gang;Chen, Tao

作者机构：

关键词： Nitric oxide; Cadmium stress; Detoxification; Transcriptome; HcERF.C3 gene; Kenaf ( Hibiscus cannabinus L.)

期刊名称：INDUSTRIAL CROPS AND PRODUCTS （ 影响因子：5.6； 五年影响因子：5.7 ）

ISSN： 0926-6690

年卷期： 2024 年 221 卷

页码：

收录情况： SCI

摘要： Nitric oxide (NO) extensively participates in regulating plant growth and abiotic stress. However, the potential effects of NO on alleviating cadmium (Cd) toxicity in kenaf (Hibiscus cannabinus L.) remain poorly understood. Herein, kenaf seedlings were pretreated hydroponically with 150 mu M sodium nitroprusside (NO donor) for 4 days, followed by 15 days of treatment with 200 mu M CdCl2 & sdot;2.5 H2O. In this study, Cd stress adversely impaired seedling growth, triggering oxidative stress and dramatically increased Cd accumulation in both shoots and roots. However, exogenous NO remarkably improved Cd tolerance in kenaf seedlings. NO pretreatment significantly enhanced the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and glutathione reductase (GR)) and the contents of proline, soluble protein, and chlorophyll b (Chl b) upon Cd exposure, which contributed to ameliorate Cd-induced oxidative injury. Under Cd stress, NO increased the contents of cell wall polysaccharides (water-soluble pectin (WSP), alkali-soluble pectin (ASP), and hemicellulose 1 (HC1)) and the abundance of negatively charged functional groups, thereby promoting Cd deposition in root cell wall. Transcriptome analysis further revealed that differentially expressed genes (DEGs) involved in cell wall biosynthesis, ROS metabolism and signal transduction, and nitrogen and sulfur metabolism played vital roles in NO-mediated Cd detoxification. ZIP1, ZIP5, ABCG8, ABCC14, CAX18, VIT4, and HIPP20 transporters were determined to be involved in Cd absorption and transport during the process of NO mitigating Cd stress. Moreover, HcERF.C3 gene was proved to have a positive impact on Cd tolerance and Cd homeostasis through virus-induced gene silencing analysis. These findings provide a deeper understanding of NO alleviating Cd phytotoxicity and a basis for improving phytoremediation.

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