In Vitro Protective Effect of Pea-Derived Peptides (PPs) via the Keap1/Nrf2 Signaling Pathway on Alpha-Gliadin-Sensitizing Peptide Induced Cacao-2 Cells

文献类型： 外文期刊

第一作者： Gao, Bing

作者： Gao, Bing;Yan, Fang;Wang, Chunfeng;Cui, Chenxu;Sun, Xuefeng;Wang, Fangyu;Li, Ning

作者机构：

关键词： antioxidant enzymes; celiac disease; free radicals scavenging; alpha-gliadin peptide; Keap1/Nrf2 pathway

期刊名称：MOLECULAR NUTRITION & FOOD RESEARCH （ 影响因子：4.5； 五年影响因子：5.6 ）

ISSN： 1613-4125

年卷期： 2024 年 68 卷 15 期

页码：

收录情况： SCI

摘要： ScopeCeliac disease (CD) is an allergic intestinal disease caused mainly by gliadin in wheat, which is widespread in the population and currently lacks effective treatment. alpha-Gliadin peptides cause cellular damage by substantially increasing cellular reactive oxygen species (ROS) levels.Methods and resultsThis study investigates the protective effect of 11 pea-derived peptides (PPs) on alpha-gliadin peptide (P31-43) treated Caco-2 cells. Results show that cells treated with PP2, PP5, and PP6 peptides significantly reduce the cell mortality caused by P31-43. Three PPs significantly reduce the P31-43-induced decrease in ROS levels to control levels, and there is no difference between them and the vitamin C (Vc) group. The results in terms of antioxidant-related enzymes show that PPs significantly decrease superoxide dismutase activity (SOD), glutathione reductases (GR), and glutathione (GSH)/oxidized glutathione (GSSG) levels, thus significantly enhancing the antioxidant level of cells. By studying the key proteins of the Kelch-like ECH-associated protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2) pathway, it is found that PPs activate the Keap1/Nrf2 signaling pathway.ConclusionThe study finds that peptides from peas can effectively alleviate alpha-gliadin peptide-induced cell damage. The discovery of these food-derived peptides provides novel potential solutions for the prevention and treatment of CD. Caco-2 Cells treated with PP2, PP5 and PP6 peptides significantly reduced the cell mortality caused by P31-43. Three PPs significantly reduced the P31-43-induced decrease in ROS levels to control levels. By studying the key proteins of the Keap1/Nrf2 pathway, it was found that PPs activate the Keap1/Nrf2 signaling pathway. Peptides from peas can effectively alleviate alpha-gliadin peptide-induced cell damage. image

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