Modulation of Keap1-Nrf2-ARE signaling pathway by oxyresveratrol, a derivative of resveratrol from grape skin

文献类型： 外文期刊

第一作者： Ma, Xinqi

作者： Ma, Xinqi;Li, Tiezhu;Ma, Xinqi;Li, Yuqiu;Lv, Chengyu;Liu, Bin;Yuan, Cuiping;Huang, Wei;Sun, Chang;Li, Tiezhu;Luo, Quan;Xiao, Yechen;Zhang, Jie

作者机构：

关键词： Oxyresveratrol; Antioxidant; Keap1-Nrf2-ARE; Molecular docking

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.318； 五年影响因子：5.846 ）

ISSN： 2212-4292

年卷期： 2022 年 50 卷

页码：

收录情况： SCI

摘要： Grape skin, one of the by-products of grape processing, is rich in oxyresveratrol and other bioactive compounds. Oxyresveratrol is a polyphenolic non-flavonoid with anti-inflammatory and anti-aging properties among other biological activities, but little research has been done on its antioxidant mechanism. This study aims to evaluate the regulation of oxyresveratrol on Keap1-Nrf2-ARE signaling pathway. Firstly, oxyresveratrol in grape skin was detected by HPLC-MS/MS, and then its interaction mechanism with Keap1 was investigated by molecular docking. The result of Western blot further confirmed that oxyresveratrol can bind with Nrf2 to induce its nuclear translocation. The Luciferase reporter gene analysis revealed the agonistic effects of oxyresveratrol on ARE. Oxyresveratrol increased not only the mRNA expression levels of ARE-mediated genes NQO1, HO-1, and GCLM, but also their protein expression levels, suggesting that oxyresveratrol inhibited H2O2-induced oxidative damage. Therefore, oxyresveratrol can be applied as a potential antioxidant by activating the Keap-Nrf2-ARE signaling pathway.

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